化学发光法测定尿酸  

Determination of Uric Acid by Chemiluminescence

作　　者：刘二保[1] 卫洪清[1,2] 

机构地区：[1]天津师范大学化学与生命科学学院,天津300074 [2]山西师范大学化学与材料科学学院

出　　处：《光谱学与光谱分析》2005年第8期1213-1215,共3页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金(20075017);天津师范大学学科建设基金资助

摘　　要：利用尿酸对碱性luminol-H2O2-Co2+化学发光体系具有较强的抑制作用, 建立了一种测定尿酸的新方法. 方法的线性范围为1.0×10-10～7.0×10-6 mol·L-1, 检测限(3σ)为1.1×10-11 mol·L-1, RSD 1.9%(n=4, cs=5.0×10-8 mol·L-1). 该法用于人体血清及尿液中尿酸的测定, 结果满意.A novel chemiluminescence method for the determination of uric acid based on its inhibitory effect on the catalysis of Co^2＋ for the oxidation of luminol by H2O2 is described. The proposed method is simple, very sensitive and inexpensive. The decrease of chemiluminescence intensity is linearly correlated with uric acid concentration. The calibration graph is linear over the range of 1.0 × 10^-10 -7.0 × 10^-6 mol·L^-1 and the detection limit is 1.1 × 10^-11 mol·L^-1. The relative standard deviation （RSD） for 5.0 × 10^-8 is 1.9% （n = 4）. The method has been successfully applied to the determination of uric acid in human urine and serum samples.

关 键 词：化学发光 尿酸 CO^2+ 

分 类 号：R446.1[医药卫生—诊断学]