抗朊蛋白单抗用于牛海绵状脑病检测和细胞型朊蛋白在中枢神经组织中的定位和分布  

Detection of Bovine Spongiform Encephalopathy Using Monoclonal Antibodies Against Prion Protein and Distribution of Cellular Prion Protein in Central Nervous System

作　　者：王小军[1,2] 王志亮[1] 鲍恩东[3] 刘雨田[1] 吴晓东[1] 

机构地区：[1]农业部青岛动物检疫所 [2]南京农业大学动物医学院,南京210095 [3]南京农业大学动物医学院

出　　处：《农业生物技术学报》2005年第4期472-476,i0001,共6页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金项目(No.30270981);科技部农业科技成果转化基金项目(No.02EFN213710657);青岛市自然科学基金项目(No.QD30270981)资助。

摘　　要：朊病毒病(又称传染性海绵状脑病)的病原是致病型朊蛋白(PrPSc),聚集在牛脑中能够引发牛海绵状脑病(BSE)。利用自制的单抗4C11和国外同类单抗进行免疫组织化学(IHC)的比较染色,结果显示单抗4C11和国际上常用的单抗在BSE阳性切片的IHC染色结果上没有显著差异;利用单抗4C11结合IHC方法检测中国山东省150例牛脑样品的实验表明,所有送检样品中IHC的检测结果均为阴性,而且IHC方法比较适合于我国BSE的检测;对中枢神经组织内细胞型PrPC的IHC定位结果显示,PrPC在牛脑组织不同部位的分布有差异。A converted form of the normal cellular prion protein （PrP^c） accumulating in the bovine brains induces bovine spongiform encephalopathy （BSE）. This so-called scrapie-associated prion protein （PrP^Sc） found in many animals and humans is the pathogen ofprion diseases. Using monoclonal antibody 4C11 （which was raised against PrP mature protein of Chinese yellow cattle） and the other two kinds of monoclonal antibodies, 6H4 and F89/160. 1.5, which were internationally used, no obvious immunohistochemistic differences were detected in all bovine brain sections of 150 samples from Shandong Province. The result showed that all bovine brain samples were completely negative. With its operating conveniently, diagnosing easily and costing lowly, immnohistochemical （IHC） staining is favorite to detect BSE. The result also showed that the distribution ofPrPc in the different parts of the central nervous system （CNS） tissues from BSE negative brains were different.

关 键 词：朊蛋白 朊病毒 单克隆抗体 牛海绵状脑病 免疫组织化学 中枢神经系统 检测结果 神经组织 细胞型 单抗 

分 类 号：Q954.67[生物学—动物学] S858.235.3[农业科学—临床兽医学]