人Norpeg蛋白的原核表达及其抗体的制备  

The Expression of Human Norpeg Protein in E.coli and Preparation of Ployclonal Antibody Against the Protein

作　　者：孙玉宁[1] 靳更林[2] 李燕[1] 寿成超[2] 

机构地区：[1]宁夏医学院生物化学教研室,银川750004 [2]北京大学临床肿瘤学院生化室,北京100034

出　　处：《宁夏医学院学报》2005年第4期256-258,F0003,共4页Journal of Ningxia Medical College

基　　金：北京市自然科学基金资助项目(7012007);北京大学肿瘤学重点学科资助项目

摘　　要：目的原核表达NorpegC端编码区并制备小鼠抗Norpeg多克隆抗体。方法将Norpeg基因C端编码区克隆进原核表达载体pGEX4T-1中,转化E.coli,以IPTG诱导重组蛋白的表达。以纯化的重组目的蛋白作为免疫原免疫小鼠,制备相应的多克隆抗体。结果成功构建原核表达载体,表达了重组蛋白并制备了小鼠抗Norpeg多克隆抗体。结论人NorpegC端编码区能够在体外大肠杆菌中获得融合表达,制备的小鼠抗Nor-peg多克隆抗体特异性较高,为下一步深入Norpeg功能研究提供了重要基础。Objective To express prokaryotically the domain C of Norpeg protein in E. coli, and prepare mouse polyclonal antibody against Norpeg. Methods The domain C of Norpeg gene was cloned into an prokaryotic expression vector pGEX4T - 1, and then the recombinant vector was transformed into E. coli . The recombinant fusion protein was expressed in DH5α under IPTG induction. Mouse polyclonal antibody against GST- Norpeg fusion protein was prepared with purified GST- Norpeg fusion protein as immunogen. Results The prokaryotic expression vector was successfully constructed and purified from E. coli. The recombinant pro- tein was expressed and polyclonal antibody against Norpeg was prepared. Conclusion Norpeg gene fragment can be expressed in E. coli, polyclonal antibody against Norpeg has higher specificity. It provides a powerful tool for further function study of Norpeg.

关 键 词：Norpeg蛋白 表达 多克隆抗体 

分 类 号：R392.11[医药卫生—免疫学]