骨髓基质细胞与人脐血基质细胞表达造血生长因子的实验研究  

The difference of mRNA expression of multiple hematopoietic growth factors between human umbilical cord blood stromal sells and bone marrow stromal cells

作　　者：刘耀[1] 张曦[1] 陈幸华[1] 彭贤贵[1] 刘林[1] 孔佩艳[1] 刘红[1] 高蕾[1] 李忠俊[1] 

机构地区：[1]第三军医大学新桥医院血液科,重庆400037

出　　处：《重庆医学》2005年第9期1321-1323,共3页Chongqing medicine

基　　金：国家自然科学基金资助项目(30070327);第三军医大学基金课题资助项目(200347)

摘　　要：目的在分子生物学水平上探讨体外培养的骨髓基质细胞与人脐血基质细胞表达TPO、GM-CSF、SCF的mRNA的能力的差异。方法收集培养28d的骨髓基质细胞与人脐血基质细胞,采用RT-PCR方法在mRNA水平上分析其造血生长因子GM-CSF、SCF、TPO的表达,比较二者表达TPO、GM-CSF、SCF等造血生长因子的mRNA能力的差异。结果体外培养的人脐血基质细胞与骨髓基质细胞均能表达:(1)TPO的mRNA,人脐血基质细胞表达能力强于同期培养的骨髓基质细胞,二者积分光密度量化比为1.57;(2)SCF的mRNA,人脐血基质细胞表达能力弱于同期培养的骨髓基质细胞,二者积分光密度量化比为0.83;(3)GM-CSF的mRNA,人脐血基质细胞表达能力弱于同期培养的骨髓基质细胞,二者积分光密度量化比为0.68。结论人脐血基质细胞和骨髓基质细胞均可以表达造血生长因子TPO、GM-CSF、SCF的mRNA,具有支持调控造血的物质基础。Objective To study the difference of mRNA expression level of hematopoietic growth factors between human umbilical cord stromal cells and bone marrow stromal cells in vitro. Methods We detected the mRNA expression level of GM-CSF, SCF, TPO of human umbilical cord blood and bone marrow stromal cells being cultured for 28d in vitro. Results Human umbilical cord blood stromal cells secrete more TPO and less SCF,GM-SCF than bone marrow stromal cells. Conclusion Human umbilical cord blood stromal cells and bone marrow stromal cells could expressi mRNA of hematopoietic growth factors such as GM-CSF, SCF, TPO, They all may regulate hematopoiesis through secreting multiple hematopoietic growth factors.

关 键 词：人脐血基质细胞 骨髓基质细胞 造血生长因子 造血调控 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]