Ras protein participated in histone acetylation-mediated cell cycle control in Physarum polycephalum  

作　　者：LI Xiaoxue LU Jun ZHAO Yanmei WANG Xiuli HUANG Baiqu 

机构地区：[1]Institute of Genetics and Cytology, Northeast Normal University,Changchun 130024, China

出　　处：《Chinese Science Bulletin》2005年第16期1721-1725,共5页

基　　金：This work was supported by the National Natural Science Foundation of China(Grant No.30370316);National Basic Research Program of China(Grant No.2005CB522404);Young Teacher Foundation of Northeast Normal University(Grant No.111494025).

摘　　要：In this paper, we demonstrate that in Physarum polycephalum, a naturally synchronized slime mold, histone deacetylase (HDAC) inhibitor Trichostatin A (TSA), arrestes the cell cycle at the checkpoints of S/G2, G2/M and mitosis exit, and influences the transcription of two ras genes Ppras1 and Pprap1, as well as the Ras protein level. Antibody neu-tralization experiment using anti-Ras antibody treatment showed that Ras protein played an important role in cell cycle checkpoint control through regulation of the level of Cyclin B1, suggesting that Ras protein might be a key factor for histone acetylation-mediated cell cycle regulation in P. polycephalum.In this paper, we demonstrate that in Physarum polycephalum, a naturally synchronized slime mold, histone deacetylase （HDAC） inhibitor Trichostatin A （TSA）, arrestes the cell cycle at the checkpoints of S/G2, G2/M and mitosis exit, and influences the transcription of two ras genes Ppras1 and Pprap1, as well as the Ras protein level. Antibody neutralization experiment using anti-Ras antibody treatment showed that Ras protein played an important role in cell cycle checkpoint control through regulation of the level of Cyclin B1, suggesting that Ras protein might be a key factor for histone acetylation-mediated cell cycle regulation in P. polycephalum.

关 键 词：蛋白质 组蛋白 乙酰化作用 细胞周期 

分 类 号：Q51[生物学—生物化学]