犬胆管腔内放射对BCL-2基因表达的影响及其与平滑肌细胞凋亡的关系  

The effect of intracholangeal radiation on the expression of BCL-2 in dogs and its relationship to apoptosis of smooth muscle cells

作　　者：何贵金[1] 鞠光 高沁怡[3] 许书河[4] 高红[5] 姜维国[6] 蒋涛[5] 戴显伟[1] 马凯[1] 

机构地区：[1]中国医科大学第二临床学院普通外科,辽宁沈阳110004 [2]辽宁省沈阳市新城子区医院外科,辽宁沈阳110013 [3]中国医科大学第一临床学院同位素室,辽宁沈阳110001 [4]中国原子能研究院同位素研究室,北京100050 [5]中国医科大学第二临床学院小儿外科试验室,辽宁沈阳110004 [6]中国医科大学第二临床学院病理科,辽宁沈阳110004

出　　处：《中国普通外科杂志》2005年第8期591-594,共4页China Journal of General Surgery

摘　　要：目的探讨γ射线对犬BCL-2基因的影响及其与胆管壁增殖平滑肌细胞凋亡的关系和意义。方法将103钯(103pd)放射性支架和普通支架分别植入犬的肝外胆管内;取出胆管标本,用免疫组化方法检测γ射线诱导的胆管壁凋亡增殖平滑肌细胞中的BCL-2基因,并检测凋亡细胞数量,计算机图象检测系统检测两组胆管腔面积。结果103pd支架组胆管组织中BCL-2基因表达较普通支架组为弱;BCL-2基因低表达组犬胆管出现增殖平滑肌细胞明显凋亡;肝外胆管无明显狭窄;BCL-2基因高表达组胆管未出现增殖平滑肌细胞明显凋亡,而肝外胆管有明显狭窄。结论实验犬BCL-2基因表达水平与细胞凋亡的发生和细胞对辐射敏感性有关;103pd放射性支架通过降低BCL-2基因表达,促进胆管增殖平滑肌细胞凋亡,从而抑制肝外胆管狭窄。Objective To study the effect of BCL-2 γ-radiation on BCL-2 gene in dogs, and its relationship and signifcane on apoptosis of proliferated smooth muscle cells of bile duct wall. Methods The ^103pd radioactivity stent （ experiment group） or ordinary stent （ control group ） was positioned into the target segment of bile duct. The injured bile duct segments were dissected free from the dogs, and BCL-2 gene in the control and r-radiation-induced apoptotic smooth mucle cells of bile duct wall was analysed by using immuno-histochemieal technique. The number of apoptotic cells was counted , and size of lumen of bile duct in both groups was measured by a computerized imaging system. Results BCL-2 gene expression was weaker in the ^103pd radioactive stent group than in the ordinary stent group. The group of dogs with low expression of BCL-2 genes showed marked apoptosis of proliferated smooth mucle cells of bile duct and there was no overt stenosis of extrahepatic bile ducts. The group that showed high expression of BCL-2 gene did not show marked apoptosisi of proliferated smooth muscle cells of bile duct, and there was marked stenosis of extrahepatic bile duct. Conclusions The expression level of BCL-2 in experimental dogs is related to the develoment of cellular apoptosis and to radiation sensitivity of the cells. ^103pd radioactive stent can reduce the expression of BCL-2 gene, promote apoptosis of proliferated smooth muscle cells of bile duct, and suppress stricture formation of extrahepatie bile duct.

关 键 词：胆管/辐射效应 Γ射线 细胞凋亡 基因 BCL-2 

分 类 号：R322.4[医药卫生—人体解剖和组织胚胎学] R812[医药卫生—基础医学]