水稻酯酶同工酶谱差异研究  

DIFFERENTIAL STUDIES ON ESTERASE LSOYME PATTERNS OF RICE VARIETIES

作　　者：李关荣[1] 刘启鑫[1] 李帮秀 

机构地区：[1]西南农业大学农学系

出　　处：《西南农业大学学报（自然科学版）》1996年第2期152-154,共3页Journal of Southwest Agricultural University

摘　　要：用聚丙烯酰胺凝胶电泳法对５种杂交稻及其亲本和１０种常规稻之间的酯酶同工酶谱研究表明，５种杂交稻种子均具９Ａ（Ｒ＿ｆ＝０，６１８）特征带，１０种常规稻绝大多数不具此带。杂交稻亲本表现较强的２Ａ（Ｒ＿ｆ＝０．６４３）和３Ａ（Ｒ＿ｆ＝０．５４３）酶带。萌发生长８～１８天的汕优６３稻苗具活性较强而稳定的１２Ａ（Ｒ＿ｆ＝０．５４３）酶带。萌发生长８～１８天的汕优６３稻苗具活性较强而稳定的１２Ａ（Ｒ＿ｆ＝０．３１２）特征带，此带在汕优２２中活性较弱，而岗优１２，岗优２２和Ⅱ优６３均无此带，这可能是汕优系列杂交稻与其它系列杂交稻的特征差异之一。By PAGE,we studied the differences of the esterase isozyme patterns of rice varieries.5 hybrids,their parents and l0 conventional rice.Restilts show that all the hybrids have a charac-teristic band marked as 9A(Rf=0.618),whereas most conventional rlce lack this band;comparedwith their respective hybrids,the parents have 2 stronger bands as 2A(Rf=0.643)and 3A(Rf=0.543);When germinating and growing for 8～18 days,the seedlings of Shangyou-63 have an evi-dent and stable characteristic band l2A(Rf=0.312).This band was also observed in the seedlingsof Shangyou-22 at the same development stage,but with much more weaker activity.However,Gangyou-l2,Gangyou-22 and Ⅱ-you-63 have no such band.Probably this is a characteristic bandof shangyou hybrid series distinguished from other hybrid series.

关 键 词：杂交稻 亲本 酯酶同功酶 

分 类 号：S511.103.2[农业科学—作物学]