纸基质室温磷光法快速测定中药材中西维因的研究  

Paper-substrate room temperature phosphorimetry for the determination of carbaryl in Chinese Herbs

作　　者：苏文斌[1] 朱若华[2] 强红[2] 王香凤[2] 谷学新[2] 

机构地区：[1]河北廊坊师范院化学系,廊坊102800 [2]首都师范大学化学系,北京100037

出　　处：《分析试验室》2005年第9期16-19,共4页Chinese Journal of Analysis Laboratory

基　　金：教育部出国留学人员基金

摘　　要：以快速定量滤纸为基质,用KI作为重原子微扰剂,建立了快速测定中药材中西维因的固体基质室温磷光(SS-RTP)分析法.其最大激发和发射波长分别为284 nm与520 nm.在优化的实验条件下,西维因在4×10-6～2×10-4 mol/L浓度范围内呈现良好的线性关系.在样品体积为10 μL时,方法的检出限为0.89 ng/斑点.所建立的方法用于中草药中西维因残留量的测定.A method for the determination of carbaryl in herbs by paper-substrate room temperature phosphorimetry （SS-RTP） was studied. The qualitative filter paper was selected as the solid substrate and 2 mol/L. KI solution was selected as the heavy atom solution. The maximum excitation and emission wavelength of carbaryl were 284 and 520 nm respectively. Under the optimal conditions, the concentration of carbaryl in the range of 4× 10^-6 - 2 × 10^-4 mol/L was linear with the phospho rescence intensity. The absolute detection limit was 0.89 ng per spot. The method was applied to the determination of carbaryl in some Chinese herb samples with satisfactory results.

关 键 词：西维因 纸基质室温 光 中草药 

分 类 号：O657.32[理学—分析化学]