河北省HIV-1流行株的env基因序列测定和亚型分析  

Subtyping and sequence analysis of the C2-V3 region of gp120 genes among HIV-1 strains in Hebei province

作　　者：赵翠英[1] 邢辉[2] 赵宏儒[1] 黄海龙[2] 刘艳芳[1] 马鹏飞[2] 李保军[1] 张玉琪[1] 王玉[2] 邵一鸣 

机构地区：[1]河北省疾病预防控制中心性病艾滋病预防监测中心 [2]中国疾病预防控制中心性病艾滋病预防控制中心

出　　处：《中华微生物学和免疫学杂志》2005年第7期533-535,共3页Chinese Journal of Microbiology and Immunology

摘　　要：目的了解河北省HIV-1亚型的分布特点和传播方式,推测流行时间,预测流行趋势. 方法采集HIV感染者的全血样品,分离外周血单核细胞(PBMC),提取前病毒DNA,使用套式聚合酶链反应(nested-PCR),扩增HIV-1的env基因的C2-V3区并进行序列测定和亚型分析. 结果对22份HIV-1感染者的样品,扩增得到了18份HIV-1 env C2-V3基因片段,经序列测定和基因分析鉴定出3种HIV-1 M亚群基因亚型,即:B′、CRF-BC和C亚型.B′亚型的组内基因离散率为7.84%±3.14%(n＝14),基因序列与云南瑞丽株rl42(泰国B亚型)相近;2株CRF-BC亚型与广西毒株的基因离散率为4.60%.与血液途径感染有关的人员均为B′(泰国B)亚型. 结论目前,在河北省发现了3种HIV-1亚型.输供血途径中B′亚型仍是主要的流行亚型,可能来源于云南吸毒人群.HIV-1 B′亚型在河北省的流行时间大约为7～9年.Objective To identify the distribution of subtypes of HIV-1 strains and their transmission patterns in Hebei province and to predict their epidemic time and epidemic trend. Methods We collected the blood samples from HIV-1 infected individuals and separated the peripheral blood mononuclear cell. The provirus DNA was extracted and amplified by nested-PCR, then the HIV-1 env C2-V3 gene fragments was sequenced and its subtypes was analyzed. Results Among 22 HIV-1 antibody positive cases, 18 HIV-1 env C2-V3 gene fragments were amplified and analyzed. Three subtypes of group HIV-1 M were identified among these cases, which are subtypes B＇ （Thailand B）, CRF-BC and C. The genetic distance of HIV-1 B＇ intersubtype is 7.84% ± 3.14% （n = 14）, their gene sequence were closely related to the subtype B in Yunnan. The genetic distance of HIV-1 CRF-BC is 4.60% to the type CRF-BC in Guangxi. Cases infected by blood transmission were all classified as subtype B＇. Conclusion There are three subtypes of HIV-1 existed in Hebei province. Blood supply is the main route of B＇ subtype transmission, with potential source of drug users of Yunnan. The epidemic time of HIV-1 B＇ subtype is about 7 to 9 years in Hebei province.

关 键 词：HIV-1 基因 亚型 序列分析 HIV-1亚型 基因序列测定 ENV基因 亚型分析 河北省 流行株 

分 类 号：R373[医药卫生—病原生物学]