Application of new tissue microarrayer-ZM-1 without recipient paraffin block  

作　　者：孟潘庆 候刚 周桂英 彭佳萍 董琦 郑树 

机构地区：[1]Cancer Institute, Second Affiliated Hospital, School of Medicine, Zhejiang University,Hangzhou 310009, China [2]Tai’an Central Hospital of Shandong, Tai’an 271000, China [3]Department of Mechanical Engineering, Advanced Technology School of Tai’an, Tai’an 271000, China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2005年第9期853-858,共6页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project (No. G1998051200) supported by the National Basic Re-search Program (973) of China

摘　　要：The ZM-1 tissue microarrayer designed by our groups is manufactured in stainless steel and brass and contains many features that make TMA (tissue microarray) paraffin blocks construction faster and more convenient. By means of ZM-1 tissue microarrayer, biopsy needles are used to punch the donor tissue specimens respectively. All the needles with the punched specimen cylinders are arrayed into the array-board, with an array of small holes dug to fit the needles. All the specimen cylinders arraying and the TMA paraffin block shaping are finished in only one step so that the specimen cylinders and the paraffin of the TMA block can very easily be incorporated and the recipient paraffin blocks need not be made in advance, and the paraffin used is the same as that for conventional pathology purpose. ZM-1 tissue microarrayer is easy to be manufactured, does not need any precision location system, and so is much cheaper than the currently used instrument. Our method’s relatively cheap and simple ZM-1 tissue microarrayer technique of constructing TMA paraffin block may facilitate popularization of the TMA technology.The ZM-1 tissue microarrayer designed by our groups is manufactured in stainless steel and brass and contains many features that make TMA （tissue microarray） paraffin blocks construction faster and more convenient. By means of ZM-1 tissue microarrayer, biopsy needles are used to punch the donor tissue specimens respectively. All the needles with the punched specimen cylinders are arrayed into the array-board, with an array of small holes dug to fit the needles. All the specimen cylinders arraying and the TMA paraffin block shaping are finished in only one step so that the specimen cylinders and the paraffin of the TMA block can very easily be incorporated and the recipient paraffin blocks need not be made in advance, and the paraffin used is the same as that for conventional pathology purpose. ZM-1 tissue microarrayer is easy to be manufactured, does not need any precision location system, and so is much cheaper than the currently used instrument. Our method＇s relatively cheap and simple ZM-1 tissue microarrayer technique of constructing TMA paraffin block may facilitate popularization of the TMA technology.

关 键 词：Tissue microarray （TMA） Construction technique Method 

分 类 号：R446.8[医药卫生—诊断学]