小麦×玉米杂交后代的蛋白质及酯酶同工酶分析  

ELECTROPHORETIC SURVEY OF PROTEINS AND ESTERASE ISOZYME IN WHEAT×MAIZE PROGENIES

作　　者：刘辉[1] 陈纯贤[1] 孙敬三[1] 王子宁[2] 郭北海[2] 方仁[2] 王景林[3] 

机构地区：[1]中国科学院植物研究所,北京100093 [2]河北省农林科学院粮油作物研究所,石家庄050031 [3]首都师范大学生物系,北京100037

出　　处：《Acta Botanica Sinica》1996年第5期357-361,共5页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金

摘　　要：以８ 个普通小麦（Ｔｒｉｔｉｃｕｍ ａｅｓｔｉｖｕｍ Ｌ．）品种为母本，２ 个栽培玉米（Ｚｅａ ｍ ａｙｓ Ｌ．）品种为父本杂交所获得的Ｆ２ 代在形态上出现了明显变异。对其籽粒进行蛋白质电泳分析，得到了如下主要结果：杂交后代的蛋白质谱带较母本有了很大的变异，主要集中在高分子量麦谷蛋白（ＨＭＷ－Ｇｌｕ）区域。杂交后代的蛋白质谱带由５ 种类型构成：１．母本型，占全部测试籽粒的２２．６％ ；２．附加型，占１４．３％ ；３．互补型，占１５．５％ ；４．杂种型，占３０．９％ ；５．缺失型，占１６．７％ 。对“矮杆早”ד紫粘”的Ｆ２ 代籽粒进行酯酶同工酶电泳分析发现，变异主要发生在ＥＳＴ－１ 区。由此看来，小麦×玉米的杂合子中玉米染色体在被排除前后。The analysis of soluble proteins and esterase isozyme in F 2 progeny grains from wheat (Triticum aestivum L.) ×maize(Zea mays L.) crosses indicated that the electrophoretic pattern of proteins and esterase isozymes was extremely different from that of their parents.Protein variation was mainly concentrated in the high molecular weight Glu (HMW Glu) zone.There were 5 kinds of protein electrophoretic patterns in the analyzed grains ,VIZ:maternal,additional,complementary,hybrid and omission type which accounted for 22.6%,14.3%,15.5%,30.9% and 16.7% of the total tested grains respectively.In the analysis of esterase pattern,some variations in progenies were also found.The variations of electrophoretic pattern of proteins and esterase isozyme indicated that a genetic material change in wheat chromosomes could be induced in the distant hybridization.

关 键 词：小麦 玉米 远缘杂交 蛋白 酯酶同工酶 分析 

分 类 号：S512.101[农业科学—作物学] S513.01