丹参粉针剂对氧化低密度脂蛋白诱导的内皮细胞表达MMP9及ICAM-1的影响  

Effect of salvia miltiorrhiza on the expression of matrix metalloproteinase 9 and intercellular adhesive moleculer-1 induced by oxidized low-density lipoprotein in human umbilical vein endothelial cells

作　　者：鞠镐[1] 程文立[2] 董晞[3] 

机构地区：[1]中日友好医院中医心肾科 [2]中日友好医院心内科 [3]中日友好临床医学研究所,北京100029

出　　处：《中日友好医院学报》2005年第4期230-232,共3页Journal of China-Japan Friendship Hospital

摘　　要：目的:研究丹参粉针剂对氧化低密度脂蛋白(ox-LDL)诱导的内皮细胞基质金属蛋白酶9(MMP9)及细胞粘附分子1(ICAM-1)表达的影响。方法:建立脐静脉内皮细胞模型,在培养液中加入ox-LDL致使脐静脉内皮损伤,分别加入130μg/ml、100μg/ml、75μg/ml、50μg/ml、25μg/ml5种浓度的丹参粉针剂溶液,观察丹参粉针剂对ox-LDL诱导内皮细胞损伤的保护作用。结果:ox-LDL可诱导脐静脉内皮细胞表达MMP9及ICAM-1,有效血药浓度内,75μg/ml、100μg/ml和130μg/ml的丹参粉针剂溶液可使MMP9及ICAM-1的表达下降。结论:丹参粉针剂可下调ox-LDL诱导的内皮细胞MMP9及ICAM-1的表达,对内皮细胞具有保护作用。Objective：To investigate the effects of salvia miltiorrhiza on the expression of matrix metalloproteinase 9 （MMP9）and intercellular adhesive moleculer-1 （ICAM-1） induced by oxidized low-density lipoprotein （ox-LDL）in human umbilical vein endothelial cells （HUVEC）.Methods：The HUVEC were incubated with 20p, g protein/ml ox-LDL and mixed with salvia mihiorrhiza for 24 hours respectively.The protein expression of MMP9 and ICAM-1 were detected by ELISA.Results：Saliva monocase significantly inhibited the expression of MMP9 and ICAM-1 of HUVEC which increased by ox-LDL.Conclusion：Saliva miltiorrhiza can significantly inhibit the ox-LDL induced expression of MMP9 in HUVEC, which might be one of the mechanisms of antiatherosclerosis.

关 键 词：丹参 氧化低密度脂蛋白 基质金属蛋白酶9 细胞粘附分子-1 

分 类 号：R285[医药卫生—中药学]