壳聚糖-生物蛋白胶用于工程化血管构建的初步研究  

Elementary study on construction of engineered blood vessel using chitosan-fibrin gel as scaffold

作　　者：徐迎新[1] 尹太[1] 李瑞新[2] 吴仕和[1] 王金晶[1] 鄂玲玲[1] 张西正[2] 李荣[1] 

机构地区：[1]解放军总医院普通外科研究所,北京市100853 [2]军事医学科学院卫生装备研究所,天津市300161

出　　处：《中国康复理论与实践》2005年第8期623-625,共3页Chinese Journal of Rehabilitation Theory and Practice

基　　金：军队医药卫生科研基金"十五"面上项目(No.01MB084)。

摘　　要：目的探讨壳聚糖管和生物蛋白胶作为工程化血管支架的可行性。方法血管内皮细胞和平滑肌细胞取自大鼠主动脉,体外扩增后,血管内皮细胞被接种在壳聚糖管的内表面;平滑肌细胞与生物蛋白胶混合后接种在壳聚糖管的外表面构建工程化血管。采用倒置显微镜观察、免疫组化染色和扫描电镜观察方法对工程化血管进行评价。结果血管内皮细胞在壳聚糖管的内壁形成连续的单层细胞层,覆盖在壳聚糖支架管腔内壁。平滑肌细胞在生物蛋白胶中成活生长,并形成三维的立体结构。结论壳聚糖生物蛋白胶可潜在地作为工程化血管支架。Objective To investigate the feasibility of construction of engineered blood vessel using chitosan tube and fibrin gel as scaffold. Methods Vascular endothelial cells and smooth muscle cells were harvested from aortas of a rat, respectively. After expansion in vitro, vascular endothelial cells were seeded onto the inner surface of chitosan tube and smooth muscle cells mixed with fibrin gel seeded onto outer surface of the scaffold to construct engineered blood vessels. Inverted microscope, immunohistochemical staining and scanning electronic microscope were used to evaluate the construct. Results Vascular endothelial cells formed monolayer and covered the inner surface of chitosan tube. Smooth muscle cells survived in the fibrin gel and grew in a 3-dimensional manner. Conclusion Chitosan-fibrin gel may be potentially used as scaffold of engineered blood vessels.

关 键 词：血管内皮细胞 平滑肌细胞 壳聚糖-生物蛋白胶支架 工程化血管 

分 类 号：R543[医药卫生—心血管疾病]