三个类核糖核酸酶基因在磷饥饿条件下的表达(英文)  

Expressions of Three Wheat S-like RNase Genes Were Differentially Regulated by Phosphate Starvation

作　　者：常胜合[1] 舒海燕[2] 童依平[1] 李滨[1] 李振声[1] 

机构地区：[1]中国科学院遗传与发育生物学研究所 [2]郑州大学生物工程学院,河南郑州450001

出　　处：《作物学报》2005年第9期1115-1119,共5页Acta Agronomica Sinica

基　　金：科技部973项目(2004CB117200);863项目(2003AA207080)资助。

摘　　要：核糖核酸酶(RNases)可以将衰老的植物组织中的核糖核酸降解释放出磷元素,使它能够运送到幼嫩部位被重新利用。许多核糖核酸酶基因的表达受磷饥饿的正调控。利用已有的EST序列,从普通小麦“小偃54”中分离了3个核糖核酸酶基因的cDNA序列。这3个基因预测的氨基酸序列与S-核糖核酸酶和S-like核糖核酸酶(类核糖核酸酶)的氨基酸序列有较高的同源性。WRN1的表达受磷饥饿和衰老的负调控,而WRN2和WRN3受磷饥饿的正调控。In plants, RNase can degrade RNA to release Pi in senescent organs, which can be reused by young organs. The expression of many S-like RNase genes were up-regulated by phosphate starvation. Using the ESTs, three S-like RNase gene cDNAs were isolated from common wheat by RT-PCR. The predicated amino acid sequences of the three sequences were found having high similarities with those S-RNases and S-like RNases in other plant species. The expression of WRN1 was down-regulated by phosphate starvation and leaf senescence, while WRN2 and WRN3 were up-regulated by phosphate starvation.

关 键 词：磷饥饿 普通小麦 类核糖核酸酶 

分 类 号：Q786[生物学—分子生物学]