RT-PCR结合限制性内切酶分析法区分NDV强弱毒株  

Detection and Typing of Newcastle Disease Virus by RT-PCR Coupled with Restriction Endonuclease Analysis

作　　者：耿捷[1] 龚振华[2] 李会荣[1] 尹燕博 黄运生[4] 罗卫[4] 张彦明[1] 

机构地区：[1]西北农林科技大学,陕西杨凌712100 [2]农业部动物检疫所,山东青岛266321 [3]山东澳兰生物工程研究所,山东青岛266515 [4]深圳出入境检验检疫局,广东深圳518045

出　　处：《中国兽医学报》2005年第5期463-466,共4页Chinese Journal of Veterinary Science

摘　　要：通过设计的特异引物,采用RT-PCR对1996年以来分离的45株国内新城疫病毒分离株进行鉴定和分析,并与其M DT实验结果进行比较,建立了RT-PCR结合Bg lⅠ内切酶分析区分新城疫强弱毒的方法。此方法操作简便、迅速,不仅能区分新城疫的强弱毒株,还可诊断AM PV-1引起的其他禽类的感染。We detected and analyzed 45 strains of Newcastle Disease Virus （NDV） in China found since 1996 by RT-PCR through designing the specific primers,compared with the results of MDT. So we set up the method of typing NDV strains by RT-PCR test coupled with restriction endonuclease analysis （REM） using BglⅠ. The method was proved to be simple and quick, not only to distinguish between virulent and low virulent isolates strains but also to diagnose the contaminations of other poultry by APMV-1.

关 键 词：新城疫病毒 强弱毒株 RT-PCR 酶切分析 

分 类 号：S852.65[农业科学—基础兽医学]