家鸡(Gallus domesticus)和鹌鹑(Coturnix coturnix)染色体核型及G带比较分析  

Comparison of the Karyotypes and G-banding Patterns between Domestic Fowl (Gallus domesticus) and Quail(Coturnix coturnix)

作　　者：徐琪[1] 陈国宏[1] 张学余[2] 王偕根 嵇宝华[1] 李碧春[1] 吴信生[1] 

机构地区：[1]扬州大学动物科学与技术学院江苏扬州,225009 [2]中国农业科学院家禽研究所,江苏扬州225003 [3]江苏省姜堰市农林局,江苏姜堰225500

出　　处：《中国兽医学报》2005年第5期547-549,共3页Chinese Journal of Veterinary Science

基　　金：国家高新技术发展计划项目(863-2001AA243082);国家自然科学基金资助项目(30170673)

摘　　要：采用外周血淋巴细胞培养法和胰酶处理法,对家鸡和鹌鹑染色体的核型和G带进行了研究。结果表明,家鸡和鹌鹑染色体数目均为2n=78,但染色体的形态存在明显差异,主要表现在NO.1、NO.4、NO.6、NO.8和Z染色体上。NO.1染色体,家鸡为m型,而鹌鹑为sm型;NO.4、NO.6、NO.8和Z染色体,家鸡分别为sm、sm、m、m型,而鹌鹑全为t型。G带分析结果显示,家鸡和鹌鹑的G带差异主要反映在NO.1、NO.2染色体上,这可能是由于臂间倒位所致。Karyotype analysis was carried out on domestic fowls and quails using the peripheral lymphocyte culture techniques and G-banding patterns were obtained with trypsin and Giemsa staining. The results showed that the domestic fowl and quail had a diploid number of 78. But their positions of centromere of macrochromosomes were different from each other. NO. 1 chromosome was metacentric, while that of quail was submetacentric. Besides, NO. 4,6,8, Z chromosome of domestic fowl were submetacentric, submetacentric, metacentric, metacentric, repectively, whereas those of quail were all telocentric. Additionally, analysis of G-banding patterns for macrochromosomes of them indicated that there was great difference between NO. 1 and NO. 2 chromosome,which can be explained by the pericentric inversions.

关 键 词：家鸡 鹌鹑 染色体 核型 G带 

分 类 号：S831.2[农业科学—畜牧学]