新基因P15RS表达产物在人胃癌细胞细胞周期中定位的研究  

Protein Localization of A Novel Gene P15RS in Cell Cycle of BGC-823 Cells

作　　者：张小勇[1] 张伟[1] 高萍[1] 常智杰[2] 孙一娜[1] 柳惠图[1] 

机构地区：[1]北京师范大学生命科学学院细胞增殖及调控生物学教育部重点实验室,北京100875 [2]清华大学生命科学与医学研究院,北京100084

出　　处：《生物化学与生物物理进展》2005年第8期771-776,共6页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助项目(39780014;30300173);细胞增殖及调控生物学教育部重点实验室资助项目~~

摘　　要：P15RS是在p15INK4b高表达的人黑色素瘤细胞MLIK6G1期中发现和克隆的新基因.研究表明,该基因可能在G1期作为增殖负调因子起作用.为了观察P15RS基因产物在细胞中的定位,构建了表达P15RS-EGFP融合蛋白的真核表达质粒pEGFP-P15RS.用绿色荧光蛋白GFP作为报告基因,以人胃癌细胞BGC-823为实验模型,将pEGFP-P15RS转染BGC-823细胞.实验表明,P15RS基因表达产物在G1期、S期和G2期均定位于细胞核内,未见在核仁中分布,M期凝缩的染色体上未见.因此,P15RS可能定位在核质中.P15RS was identified as a novel gene cloned from the human melanoma cell model MLIK6 overexpressing p15^INK4b. P15RS has been reported as a negative regulator of cell proliferation in G1 phase. To investigate the localization of P15RS, EGFP-P15RS, a fusion protein with EGFP reporter was constructed and overexpressed in BGC-823 cells. The results showed that EGFP-P15RS protein was distributed in nuclear in interphase （G1, S, G2 phase） of BGC-823 cells. The EGFP-P15RS protein was observed not to localize in chromosome in M phase, suggesting that P15RS might be located in karyoplasm.

关 键 词：P15RS 人胃癌细胞 GFP 细胞定位 

分 类 号：Q291[生物学—细胞生物学]