ONO-AE-248诱发的中性粒细胞非凋亡、非坏死性死亡  

Non-apoptotic, non-necrotic death of neutrophils induced by EP_3 agonist——ONO-AE-248

作　　者：刘佳佳[1] 张姿[1] 何浩[1] 李昕[1] 何延政[2] 

机构地区：[1]泸州医学院分子生物学实验室,四川泸州646000 [2]泸州医学院附属医院血管外科,四川泸州646000

出　　处：《细胞与分子免疫学杂志》2005年第5期541-543,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助(No.30371305);四川省杰出青年基金资助(No.川科基[2001]2#);四川省学术和技术带头人培养基金资助(No.川人办发[2003]274#)

摘　　要：目的:观察前列腺素E2受体亚型3(EP3)激动剂ONO-AE-248对中性粒细胞的影响。方法:运用电子显微镜、MTS法、RT-PCR和流式细胞术等技术,对中性粒细胞的形态变化、生物学活性及凋亡进行观察和评估,并对EP3受体进行分析。结果:中性粒细胞存在EP3受体基因的表达,以5mmol/L的ONO-AE-248作用12h后,其多叶核融合为单叶核,核膜起泡、破裂,核质溢出,细胞死亡;但其快速死亡不具有细胞凋亡和坏死的形态特征且需要能量(ATP)供应。结论:ONO-AE-248所致这种新型细胞主动死亡的形式,可能为“非凋亡性程序化细胞死亡”的一种,这对于重新认识和定位中性粒细胞在非特异性免疫中的作用和地位提出了新的思路。AIM： To investigate the effect of EP3 agonist--ONO-AE-248 on neutrophils. METHODS： Electron microscopy, MTS assay, RT-PCR and FACS were used to observe the morphology, viability, EP3 receptor expression and apoptosis of neutrophils treated with ONO-AE-248. RESULTS： EP3 receptor was expressed on neutrophils. ONO-AE-248 rapidly caused a unique form of neutrophil death, showing morphological changes of nucleus, including fusion of the Iobules, blebbing and rupture of the nuclear membrane, which were different from typical morphological changes of apoptosis and necrosis. The death was dependent on ATP. CONCLUSION： The death of neutrophils caused by ONO-AE-248 is likely to be one of the forms of “non-apoptotic programmed cell death”.

关 键 词：ONO-AE-248 非凋亡性程序化细胞死亡 中性粒细胞 凋亡 坏死 

分 类 号：R329.12[医药卫生—人体解剖和组织胚胎学]