正常人IgG类天然抗角蛋白自身抗体反应特性分析  

Reactivity of IgG class of anti-keratin autoantibodies in healthy human sera

作　　者：党育平[1] 李巍[1] 李承新[1] 夏汝山[1] 孙林潮[1] 刘玉峰[1] 

机构地区：[1]第四军医大学西京医院全军皮肤性病中心,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2005年第5期622-624,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：全军医药卫生科研基金重点项目(No.01Z086);国家自然科学基金资助项目(No.30330510;30300318)

摘　　要：目的:分析纯化前后正常人血清IgG类抗角蛋白自身抗体对角蛋白的反应型式,探讨血清非IgG成分对自身AKautoAbs结合特性的影响。方法:用间接ELISA检测正常人血清IgG类AKautoAbs纯化前后的滴度,Westernblot检测全血清和纯化IgG对自身角蛋白的反应特征。结果:纯化前不同个体血清中IgG类AKautoAb的滴度差异很大,反应型式高度异质;纯化后IgG的AKautoAb滴度较纯化前明显升高。Westernblot分析显示纯化后的IgG类AKautoAb识别的角蛋白成分条带增加,反应性增强,且个体之间的反应型式趋于一致。结论:血清中IgG类AKautoAbs的反应型式受一些非IgG成分不同程度的影响;个体之间高度一致的反应型式提示产生IgG类AKautoAbs的B细胞在发育过程中可能受正常机体一组自身抗原(角蛋白)的选择,而与机体接触的外来抗原无关。AIM： To analyze the reaction patterns of lgG class of anti-keratin autoantibodies （AK auto Abs） in normal human sera before and after purification, and to explore the effects of serum non-lgG components on the reaction patterns of IgG class of AK auto Abs. METHODS： Titers of IgG AK auto Abs in healthy human sera before and after purification were measured by indirect ELISA, and the reaction patterns of the AK auto Abs to a group of keratins with different relative molecule mass （ Mr ） were analysed by Western blot. RESULTS： Titers of purified IgG class of AK auto Abs were higher than those of unpurified sera. Western blot analysis showed purified AK auto Abs recognized more keratins with stronger avidity than unpurified AK auto Abs. The reaction patterns of AK auto Abs from different individuals tended to be homogenous after purification of AK auto Abs. CONCLUSION： The reaction patterns of serum IgG class of AK auto Abs are influenced by some non-lgG components in sera. The homogeneity of reaction patterns of AK auto Abs from different individuals indicated that B cells producing IgG class of AK auto Abs may be selected by a set of conserved self-antigens, and this process may have no relationship with an individual＇s exposure to foreign antigens.

关 键 词：角蛋白 自身抗体 反应型式 

分 类 号：R329.12[医药卫生—人体解剖和组织胚胎学]