骨形成蛋白2基因修复兔桡骨缺损的实验研究  

DIRECT BONE MORPHOGENETIC PROTEIN 2 GENE THERAPY FOR REPAIRING SEGMENTAL RADIAL DEFECT IN RABBITS

作　　者：李建军[1] 白伦浩[1] 孙鸿斌[2] 韩冬[3] 顾稼祥[2] 王欢[1] 段景柱[1] 徐莘香[4] 

机构地区：[1]中国医科大学附属二医院骨科,沈阳110003 [2]吉林大学中日联谊医院手外科 [3]锦州医学院附属一医院骨科 [4]吉林大学第一医院骨科

出　　处：《中国修复重建外科杂志》2005年第9期725-728,共4页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：吉林省科委科研基金资助项目(20010110)~~

摘　　要：目的观察携带人骨形成蛋白2(bonemorphogeneticprotein2,BMP-2)基因的腺病毒载体(adenoviruscarryingBMP-2gene,Ad-BMP-2),通过纤维蛋白凝胶与牛松质骨支架(bovinecancellousbone,BCB)复合,修复骨缺损的效果。方法将60只新西兰大耳白兔随机分为4组,每组15只。制成双侧桡骨中段1.5cm骨缺损模型,采用4种材料植入修复。A组Ad-BMP-2+BCB;B组重组BMP-2+BCB;C组携带β-半乳糖酐酶基因的腺病毒对照载体(adenoviruscarryingβ-galgene,Ad-Lacz)+BCB;D组单纯BCB支架。修复术后各组于4、8和12周各处死动物5只取材,行X线片、组织学、生物力学和免疫组织化学染色检查。结果A、B两组骨缺损均得到了修复,但术后各时间点,A组在成骨活跃程度、新生骨量、力学强度及BMP-2表达等方面均明显优于B组;C、D两组均无新骨形成。结论BMP-2直接基因治疗,操作简便、骨诱导能力强,是修复节段性骨缺损的有效方法。Objective To study the effect of direct bone morphogenetic protein 2 （BMP-2） gene therapy mediated by adenovirus on repairing bone defect. Methods The radial defect models were made on 60 rabbits, which were evenly divided into 4 groups randomly. The 4 groups were treated with different materials： group A, adenovirus carrying BMP-2 gene （Ad-BM- 2） plus bovine cancellous bone （BCB）; group B, reconstructed BMP-2 plus BCB; group C, Ad-Lacz plus BCB; and group D, only BCB scaffolds. The X-ray, histological examination, biomechanics analysis, and immunohistoehemical staining were made 4, 8, and 12 weeks after the operation. Results Group A gained better effect in the volume of new bones, the anti bending intensity of the healing bone, and the expression of BMP-2 than those of group B. The defect in groupA was healed. No new hones were observed in group C and group D. Conclusion Direct BMP-2 gene therapy is easy to perform and has very strong osteoinduction ability. It is a good method to repair segmental bone defects.

关 键 词：骨形成蛋白2 基闪治疗 骨缺损 修复 

分 类 号：R683[医药卫生—骨科学]