大鼠蛛网膜下腔出血脑基底动脉内皮素受体mRNA表达的变化  

THE CHANGES OF ENDOTHELIN-A RECEPTOR mRNA IN THE CEREBRAL BASILAR ARTERY OF RATS WITH SUBARACHNOID HEMORRHAGE

作　　者：林雪群[1] 黄海鹰[2] 祝高春[1] 石嘉庆[1] 戴育成[3] 熊建忠[1] 

机构地区：[1]江西医学院脑血管研究所 [2]江西医学院第三附属医院脑外科 [3]江西医学院血液病研究所,南昌330006

出　　处：《解剖学报》2005年第4期400-402,共3页Acta Anatomica Sinica

基　　金：卫生部科学基金(98-1-337);江西省自然科学基金资助(0440062)

摘　　要：目的探讨实验性蛛网膜下腔出血诱发脑血管痉挛时,内皮素受体-A(ETA)基因在大鼠脑基底动脉的表达变化及在诱发脑血管痉挛中的作用。方法56只Wistar大鼠随机分为正常组、蛛网膜下腔出血组(2、3、7、14d)、生理盐水组和假手术组,应用逆转录-聚合酶链反应(RT-PCR),以β-actin为内参照基因,分别检测各组实验动物脑基底动脉ETAmRNA的表达变化。结果蛛网膜下腔出血后2、3d组脑基底动脉ETAmRNA表达较正常组明显增高,7d组仍增高,14d组趋于正常。结论ETA在蛛网膜下腔出血诱发脑血管痉挛的病理生理过程中可能起重要作用。Objective To detect the changes of Endothelin-A receptor mRNA in the cerebral basilar artery of rats with experimental subarachnoid hemorrhage-induced cerebral vessel spasm as well as its effect. Methods Fifty-six Wistar rats were randomly divided into normal, experimental, sham-operated and saline-control groups. The experimental group was subdivided into subarachnoid hemorrhage（SAH）2,3,7 and 14 d groups. Reverse transcription polymerse chain reaction was used to study the changes of Endothelin-A receptor mRNA level in the cerebral basilar artery of rats with experimental subarachnoid hemorrhageinduced cerebral vessel. Results ET-A receptor mRNA level of basilar artery in SAH2, 3 d and 7 d group was higher obviously than that of normal group, sham-operated and saline-control groups. That of 14 d group was approximate to normal group. Conclusion ET-A receptor may play an important role in pathogenesis of SAH-induced cerebral vessel spasm.

关 键 词：蛛网膜下腔出血 脑血管痉挛 内皮素受体-A 逆转录-聚合酶链反应 大鼠 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学]