1997-2002年山东省HIV-1流行株env基因序列测定和亚型分析  

SUBTYPES AND SEQUENCE ANALYSIS OF C2-V3 REGION OF env GENES AMONG HIV-1 STRAINS IN SHANDONG PROVINCE DURING 1997-2002

作　　者：宋旭霞[1] 钱冬萌[1] 傅继华 方荣[1] 吴虹[1] 王斌[1] 

机构地区：[1]青岛大学医学院微生物学教研室,山东青岛266021 [2]山东省CDC艾滋病中心

出　　处：《齐鲁医学杂志》2005年第5期385-388,共4页Medical Journal of Qilu

基　　金：山东省优秀中青年科学家奖励基金资助项目(编号:1997BB2CBA1)

摘　　要：①目的对1997～2002年间山东地区HIV-1各亚型分离株进行基因分型,了解各亚型的分布及其与传播途径的相互关系.②方法采集1997～2002年间山东省38例HIV-1感染者的抗凝全血标本,用巢式PCR技术扩增外周血单个核细胞中HIV-1前病毒env基因C2～V3区,并对C2～V3区的核苷酸序列进行测定和分析.③结果山东省38例HIV-1分离株中存在亚型和重组毒株4种(A、B、C、A/E).其中A亚型5株,B亚型22株(其中包括泰国B亚型11株),C亚型10株,A/E重组毒株1株.各亚型内的离散率有差异,A、B、C亚型内基因离散率分别为1.5%、8.6%和1.9%.在性乱人群和静脉吸毒人群中以A亚型和C亚型为主;在职业献血和不安全血制品使用者中以B亚型为主.④结论 1997～2002年间山东省HIV-1流行特点为A、B、C亚型共存,伴有重组毒株;传播途径复杂,基因变异较大,B亚型仍然为主要流行株.Objective To identify the subtype of HIV-1 isolated in Shandong Province during 1997-2002, and to analyze the relationship between distribution of HIV-1 subtypes and transmission routines. Methods HIV-1 env C2-V3 DNA fragments were amplified by nested-PCR from PBMs obtained from 38 HIV-1 infectors from Shandong Province during 1997-2002, the C2-V3 region were sequenced. Results Four subtypes or circulating recombinant forms （CRFs） of HIV-1 were found in Shandong Province： subtype A （5 strains）, B （22 strains, 11 strains of Thai B or B＇ncluded）, C （10 strains） and A/E （1 strain）. The gene divergences inside the subtypes were different, with 1.5%, 8.6% and 1.9% in subtype A , B and C, respectively. Subtypes A and C were predominant among IDUs and heterosexuals, while subtype B among commercial blood donors and receptors of insecure blood products. Conclusion The epidemic characteristics of HIV-1 in Shandong Province during 1997-2002 were as follows： A, B and C subtypes coexisted; the transmission routines were complicated; the genomes varied obviously; subtype B was still the primary epidemic stain,

关 键 词：HIV-1 基因 ENV 序列分析 

分 类 号：R372[医药卫生—病原生物学]