骨宝液在去卵巢大鼠骨质疏松症中抑制骨转换作用的实验研究  

The experimental study of the effects of Gubaoye(骨宝液)on osteoporosis in ovariectomized rats by inhibiting the bone turnover

作　　者：张兆华[1] 陈志维[1] 陈逊文[1] 罗汉文[1] 

机构地区：[1]广州中医药大学附属佛山市中医院骨科,广东佛山528000

出　　处：《中国骨伤》2005年第8期473-476,共4页China Journal of Orthopaedics and Traumatology

基　　金：广东省中医药管理局科研课题(编号:1040177)

摘　　要：目的:探讨骨宝液对去卵巢大鼠骨质疏松症中抑制骨转换作用。方法:60只大鼠去卵巢诱导骨质疏松症,随机分为正常对照组、模型组、尼尔雌醇组、骨宝液低、中、高剂量组。正常对照组、模型组以0.9%生理盐水每天10ml/kg,尼尔雌醇组以尼尔雌醇每2周1mg/kg,其他三组每天分别以骨宝液5、101、5ml/kg的剂量灌胃给药3个月后,以比色法测定其血清钙(s-Ca)、骨碱性磷酸酶(b-AKP)和血清抗酒石酸酸性磷酸酶(s-TRAP)的活性,竞争放射免疫法测定血清中骨钙素(s-BGP)的含量;双能X线骨密度仪(DEXA)测定股骨及腰椎的骨密度;三点弯曲试验法测定骨生物力学;扫描电镜进行骨小梁形态计量学分析。结果:骨宝液组与其他组比较,s-TRAP水平降低,腰椎骨密度增加,股骨抗弯抗拉能力改善,骨小梁形态改善,骨小梁平均宽度(MTT)与骨小梁面积百分比(TS%)显著增加。结论:骨宝液对去卵巢大鼠骨质疏松症具有防治作用,其机制可能与抑制骨转换有关。Objective：To explore the effects of Gubaoye on osteoporosis in ovariectomized rats by inhibiting the bone turnover. Methods： Sixty rats were induced to osteoporosis by ovariectomy, and randomly divided into six groups（ten rats in each group） ：A control group, a model group, a Nilestriol group, a low-dose-Gubaoye group,a midcUe-Gubaoye group and a high-dose-Gubaoye group. The control and model groups were fed with 0.9% saline 10 ml/kg once a day. The Nlestriol group was fed with 1 mg/kg. The other three groups were fed with Gubaoye at the dose of 5,10,15 ml/kg once a day respectively. After fedding for 3 months, the concentration of calcium （s-Ca）,tartrate resistant acid phosphatase in serum （s-TRAP）and alkaline phosphatase in bone （b-AKP）were measured by colorimetric method. The concentration of osteocalcin in serum （s-BGP）was measured by competitive radio immunologic method. Bone mineral density （BMD） of femur and lumbar of rats was determined by Dual Engergy X-ray Absorbtiometry （DEXA）. Biomechanical characteristics of femur were measured by three point bending test. Histomorphometry of bone trabecula was analysed by scanning electron microscope. Results： As compared with the other groups, In the ubaoye groups, the level of s-TRAP was decreased;The bone density of lumbar vertebra was increased; The resistance of bending and compression of furor of rats was enhanced; The form of bone trabecula was impoved and MTT and trabeculae square percentage （TS%） were obviously increased, as compared with other groups. Conclusion： Gubaoye has anti-osteoporotic effects on ovariectomized rats by inhibiting the bone turnover.

关 键 词：卵巢切除术 骨质疏松 中药 去卵巢大鼠 骨质疏松症 骨转换 中抑制 骨宝 双能X线骨密度仪 实验研 

分 类 号：R681[医药卫生—骨科学] R338.3[医药卫生—外科学]