Electrochemical Detection of Single A-G Mismatch Using Biosens-ing Surface Based on Gold Nanoparticles  

作　　者：Ren-Yun Zhang Xue-Mei Wang Sheng-Jin Gong Nong-Yue He 

机构地区：[1]Chien-Shiung Wu Laboratory, National Lab of Molecular and Biomolecular Electronics, Department of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.

出　　处：《Genomics, Proteomics & Bioinformatics》2005年第1期47-51,共5页基因组蛋白质组与生物信息学报（英文版）

基　　金：This work was supported by the National Natural Sci—ence Foundation of China(No.20205001 and No.60121101).

摘　　要：The study of small drug molecules interacting with nucleic acids is an area of intense research that has particular relevance in our understanding of relative mechanism in chemotherapeutic applications and the association between genetics （including sequence variation） and drug response. In this contribution, we demonstrate how the sequence-specific binding of an anticancer drug Dacarbazine （DTIC） to single base （A-G） mismatch could be sensitively detected by combining electrochemical detection with biosensing surface based on gold nanoparticles.The study of small drug molecules interacting with nucleic acids is an area of intense research that has particular relevance in our understanding of relative mechanism in chemotherapeutic applications and the association between genetics （including sequence variation） and drug response. In this contribution, we demonstrate how the sequence-specific binding of an anticancer drug Dacarbazine （DTIC） to single base （A-G） mismatch could be sensitively detected by combining electrochemical detection with biosensing surface based on gold nanoparticles.

关 键 词：single base mismatch DTIC ELECTROCHEMISTRY gold nanoparticles BIOSENSOR 

分 类 号：R979.1[医药卫生—药品] O646[医药卫生—药学]