黄酮对脑缺血-再灌流损伤保护作用的实验研究  

Protective effects of anthoxanthin on cerebral ischemia-reperfusion injury

作　　者：杨洁红 别晓东[2] 杜月光[1] 樊守艳[1] 郭莹[1] 韩进[1] 万思佳[1] 潘远江[2] 万海同[1] 

机构地区：[1]浙江中医学院心脑血管病研究所,浙江省杭州310053 [2]浙江大学医学院附属第二医院

出　　处：《中华急诊医学杂志》2005年第9期735-737,共3页Chinese Journal of Emergency Medicine

基　　金：国家自然科学基金资助项目(30371789);浙江省自然科学基金项目(Z204424;302608)

摘　　要：目的探讨养阴通脑颗粒中黄酮对脑缺血-再灌流损伤的保护作用。方法制作SD大鼠脑缺血-再灌流损伤模型,随机分假手术对照组、脑缺血-再灌流损伤组和黄酮组及维脑路通对照治疗组3组,观察各组脑组织前列环素(PGI2)含量、脑组织超氧化物岐化酶(SOD)活性和丙二醛(MDA)含量、血浆组织型纤溶酶原激活物(t-PA)活性及其抑制物(PAI)活性和脑组织ET-1基因表达等指标的变化。结果黄酮可显著提高脑组织PGI2含量;调节血浆t-PA与PAI的活性;显著降低脑组织MDA含量,提高脑组织SOD活性,抑制脑缺血脑皮层ET-1基因表达。结论黄酮具有抗凝、提高纤溶活性和提高细胞抗氧化等多种作用,黄酮对脑缺血-再灌流损伤具有保护作用。Objective To study protective effects of anthoxanthin on cerebral ischemia-reperfusion injury. Methods The cerebral ischemia-reperfusion injury models in SD rats were established and rats were divided into 4 groups at random： sham operation group, cerebral ischemia-reperfusion group, anthoxanthin group and Weinaolutong group. The contents of PGI2, TXA2, superoxide dismutase （SOD） and malonyl dialdehyde （MDA）, and the changes of activated target of tissue plasminogen activator （t-PA） and its inhibitor （PAl） in blood plasma were measured. Results The anthoxanthin can increase the content of PGI2 and decreased the TXA2. It can regulate t-PA and PAI in plasma, decrease MDA and increase the SOD activity in cerebral tissue. Conclusion Anthoxanthin plays a role in antieoagulation, strengthening plasminogen activator and promoting the anti-oxidation of cell.

关 键 词：脑缺血 再灌流损伤 黄酮 有效部位 中医药 脑缺血-再灌流损伤 保护作用 组织型纤溶酶原激活物(t-PA) 实验研 SOD活性 

分 类 号：R285[医药卫生—中药学]