HPLC法比较2种银杏外种皮多糖的相对分子质量及含量  

Comparison of molecular weight and concentrations of polysaccharides from Ginkgo biloba by HPLC

作　　者：任莉[1] 许爱华[1] 鲁萍[2] 

机构地区：[1]扬州大学医学院 [2]扬州大学化学化工学院,扬州225002

出　　处：《中国新药杂志》2005年第8期1027-1029,共3页Chinese Journal of New Drugs

基　　金：江苏省科技厅社会发展基金资助项目(BS2000086)

摘　　要：目的:采用HPLC法比较2种银杏外种皮多糖的相对分子质量及含量。方法:从银杏外种皮中提取出多糖,分别于常温下贮藏5年和3年后测定并比较其相对分子质量和含量。以右旋糖酐为标样,采用HPLC法,PLaquagel-OHMIXED(300mm×7.5mm,8μm)色谱柱,柱温25℃,示差折光检测器,流动相为超纯水,流速1.0mL·min-1。结果:贮藏5年和3年的2种银杏外种皮多糖的平均相对分子质量分别为10825.3(RSD=0.45%)和11062.5(RSD=0.78%);其多糖含量分别为66.8%(RSD=5·.9%)和68.7%(RSD=3.4%)。结论:在常温下,银杏外种皮多糖随着贮藏时间的延长,其相对分子质量和含量会发生变化。Objective：To compare the molecular weight and concentrations of polysaccharides from two species of exocarps of Ginkgo biloba by HPLC. Methods ： Following after the storage of polysaccharide extracts from 2 species of Ginkgo bUoba exocarps at room temperature for 5 and 3 years, the molecular weight and concentrations of polysaccharides were measured and compared by HPLC. The HPLC condition was as follows ： PLaquagel-OH MIXED column（300 mm × 7.5 mm, 8μm） with column temperature 25℃, pure aqueous solution as mobile phase with a flow rate of 1.0 mL·min^-1 , and detection by RID. The standard curves were made using dextran. Results：The average molecular weight and concentrations of polysaccharides stored for 5 years and 3 years were 10825.3 （ RSD = 0.45 % ） vs. 11062.5 （ RSD = 0.78 % ） and 66.8 % （ RSD = 5.9 % ） vs. 68.7 % （ RSD = 3.4 % ）, respectively. Conclusion ： As the storage period extends, the molecular weight and concentration of Ginkgo biloba exocarp polysaccharides will be changed at room temperature.

关 键 词：银杏 多糖 HPLC 相对分子质量 含量 

分 类 号：R282.71[医药卫生—中药学] R284.1[医药卫生—中医学]