甘氨酰谷氨酰胺对粤黄鸡免疫功能的影响  

Effect of Glycyl-glutamine on Immune Functions of Yuehuang Chicken

作　　者：朱晓彤[1] 崔志英[1] 高萍[1] 江青艳[1] 束刚[1] 徐平稳[1] 傅伟龙[1] 

机构地区：[1]华南农业大学动物科学学院,广州510642

出　　处：《畜牧兽医学报》2005年第9期956-959,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：广东省科技计划资助项目(2003C20204)

摘　　要：This study aimed to investigate the influence of Glycyl-glutamine on the development of immune functions of Yuehuang chicken, and to investigate its effect on proliferation and secretion of chicken lymphocytes. In experiment one ,360 1-day-old Yuehuang chicken were randomly divided into 4 groups, with 90 birds in each group. Group 1 served as control, while group 2 to 4 were respectively supplemented ad libitum with 100,500 and 1 000 μg/L Gly-Gln via drinking water. On 45 and 80 days of age, 18 birds in each group were slaughtered and the bursa of Fabricius , spleen and thymus were weighted and blood samples were collected. At the same times, 3 birds in each group were selected and lymphocytes from blood and spleen were collected for measuring proliferation and secretion of IL-2 and IL-6. Experiment two was conducted to test the proliferation and secretion of lymphocytes when cultured with the addition of Gly-Gln. Lymphocytes from blood and spleen were isolated from 8-week-old chicken without treatment of Gly-Gln, and culture in media added with 0, 0.6,1.2 and 2.4 mmol/L Glycyl-glutamine solution respertively. The results showed : (1) Supplementation of Gly-Gln had no significant effect on the development of Fabricius Bursa; but Gly-Gln significantly decreased the thymus indices at 1 000 μg/L level and improved the spleen indices at 500 μg/L level in 80-day-old chickens. (2) The additions of both 500 μg/L and 1 000 (μg/L ) Gly-Gln significantly increased concentrations of IL-2 and IL-6 in the sera of Yuehuang chicken on 45 and 80 days of age, and enhanced blood lymphocyte proliferation , but inhibited spleen lymphocyte proliferation in 45-day-old chicken.(3) The addition of Gly-Gln at concentration of 2.4 mmol/L in the culture medium significantly enhanced lymphocyte proliferation ,but Gly-Gln did not affect the secretion of IL-2 and IL-6 form cultured lymphocytes.This study aimed to investigate the influence of Glycyl-glutamine on the development of immune functions of Yuehuang chicken, and to investigate its effect on proliferation and secretion of chicken lymphocytes. In experiment one ,360 1-day-old Yuehuang chicken were randomly divided into 4 groups, with 90 birds in each group. Group 1 served as control, while group 2 to 4 were respectively supplemented ad libitum with 100,500 and 1 000 μg/L Gly-Gln via drinking water. On 45 and 80 days of age, 18 birds in each group were slaughtered and the bursa of Fabricius , spleen and thymus were weighted and blood samples were collected. At the same times, 3 birds in each group were selected and lymphocytes from blood and spleen were collected for measuring proliferation and secretion of IL-2 and IL-6. Experiment two was conducted to test the proliferation and secretion of lymphocytes when cultured with the addition of Gly-Gln. Lymphocytes from blood and spleen were isolated from 8-week-old chicken without treatment of Gly- Gln, and culture in media added with 0, 0.6,1.2 and 2.4 mmol/L Glycyl-glutamine solution respertively. The results showed ： （1） Supplementation of Gly-Gln had no significant effect on the development of Fabricius Bursa; but Gly-Gln significantly decreased the thymus indices at 1 000 μg/L level and improved the spleen indices at 500 μg/L level in 80-day-old chickens. （2） The additions of both 500 μg/L and 1 000 μg/L Gly-Gln significantly increased concentrations of IL-2 and IL-6 in the sera of Yuehuang chicken on 45 and 80 days of age, and enhanced blood lymphocyte proliferation , but inhibited spleen lymphocyte proliferation in 45-day-old chicken. （3） The addition of Gly-Gln at concentration of 2.4 mmol/L in the culture medium significantly enhanced lymphocyte proliferation , but Gly-Gln did not affect the secretion of IL-2 and IL-6 form cultured lymphocytes.

关 键 词：甘氨酰谷氨酰胺 鸡 免疫器官 淋巴细胞 IL-2 IL-6 

分 类 号：S852.4[农业科学—基础兽医学]