维生素B_(12)与DNA相互作用的电化学研究  

Electrochemical Studies of Interaction Between DNA and Vitamin B_(12)

作　　者：包晓玉[1] 党元林[1] 陈欣[1] 陈建国[1] 

机构地区：[1]南阳师范学院化学系,南阳473061

出　　处：《应用化学》2005年第9期993-996,共4页Chinese Journal of Applied Chemistry

基　　金：河南省教委自然科学基础研究立项课题(20021800002)

摘　　要：着重研究了维生素B12与DNA在pH=4.87条件下相互作用的电化学行为。DNA的存在能导致维生素B12还原峰电流降低。通过测定DNA引入前后的一些电化学参数,推测维生素B12与DNA在该条件下结合生成了一种非电活性的超分子化合物。针对该类型体系,推导出了一系列的方程求得该超分子化合物的组成为1∶1,结合常数β=1.6×105,加入DNA后,VB12峰电流降低,据此可以测定DNA。The present paper covers the electrochemical behavior of the interaction of Vitamin B12 with DNA at pH 4. 87. After adding DNA in the Vitamin B12 solution, the reduction current of Vitamin B12 decreases. From the determination of the electrochemical parameters（ charge transfer coefficient ot and standard electron transfer rate constant k.） in the absence and presence of DNA, it was found that an electrochemical inactive supermolecular complex DNA-VB12 was formed. A series of equations was deduced for this kind of system. The equilibrium constant of this complex was calculated to be 1.6 × 10^5 mol/L. The decrease in peak current of VB12 is proportional to DNA concentration and it can be used to determine DNA concentration.

关 键 词：维生素B12 DNA 循环伏安法 紫外可见吸收光谱法 

分 类 号：O641.3[理学—物理化学]