益骨胶囊对去卵巢骨质疏松大鼠骨组织Ⅰ型胶原mRNA表达的影响  

Effects of Benefiting-bone Capsule on Expression of mRNA of Type Ⅰ Collagen in Bone Tissue of the Ovariectomized Rats

作　　者：张荣华[1] 彭勇 杨丽[1] 蔡宇[3] 朱晓峰[1] 

机构地区：[1]暨南大学附属第一医院,广东广州510630 [2]广东省汕头市出入境检验检疫局,515000 [3]暨南大学药学院,广东广州510632

出　　处：《时珍国医国药》2005年第10期941-943,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30472274);广东省自然科学基金重点项目(No.04105837);广东省社会发展计划重点引导项目(No.2004B33001004)

摘　　要：目的:观察益骨胶囊治疗用药对去卵巢骨质疏松大鼠骨组织Ⅰ型胶原mRNA表达的影响,以探讨益骨胶囊治疗骨质疏松症的可能机理。方法:36只10月龄SD雌性大鼠随机抽取12只为假手术组,剩余24只待手术造模后随机分为2组:模型组、益骨胶囊组;以双侧卵巢切除法复制骨质疏松模型;采用RT-FQ-PCR方法检测骨组织Ⅰ型胶原mRNA的表达。结果:益骨胶囊组骨组织Ⅰ型胶原mRNA表达明显高于模型组(P<0.05),与假手术组相近(P>0.05)。结论:益骨胶囊能促进骨组织Ⅰ型胶原mRNA基因表达。Objective：The research was to investigate the effects of Benefiting-bone capsule （BBC） on the expression of mRNA of Type Ⅰ collagen in bone tissue of the ovariectomized rats to further explain the mechanism of BBC on preventing and treating osteoporosis, Methods： 12 rats were randomly selected from 36 female SD rats aged 10 months which were equally divided into group of sham operation with normal physiological saline. The left 24 rats were randomly divided into 2 groups after ovariectomy：group of osteoporotic ,animal model with normal physiological saline, treated group of osteoporotic animal model with BBC, Each group included 12 rats. Ovariectomy was used to establish the osteoporotic animal model and the expression of Type Ⅰ collagen mRNA on gene level in hone tissue was detected by real time fluorescence quantitative PCR（FQ-PCR） method. Results：The expression of Type Ⅰ collagen mRNA on gene level in bone tissue of group of osteoporotic animal model with BBC was higher than group of osteoporotic animal model （P〈0.05） ,which was closed to group of sham operation. Conclusion：BBC can improve the expression of bone tissue Type Ⅰ collagen on mRNA level of the rats performed by ovariectomy.

关 键 词：益骨胶囊 骨质疏松症 大鼠 Ⅰ型胶原 

分 类 号：R285.5[医药卫生—中药学] R269[医药卫生—中医学]