黄伞菌株中三磷酸甘油醛脱氢酶基因片段的分离鉴定与保守性验证  

Cloning of the Fragment of Glyceraldehyde-3-Phosphate Dehydrogease(GPD)Gene from Pholiota adiposa(Fr.) Quél Strain and Verifying the Conservativeness

作　　者：季哲[1] 李玉祥[1] 赵明文[1] 潘迎捷[2] 

机构地区：[1]南京农业大学农业部农业环境微生物工程重点开放实验室,南京210095 [2]上海水产大学,上海200090

出　　处：《中国食用菌》2005年第5期53-55,共3页Edible Fungi of China

摘　　要：本研究以黄伞菌丝cDNA为模板,设计简并引物,经PCR扩增出两条片段SL1和SL2,经过回收、重扩增、克隆与鉴定后进行测序分析,结果发现SL1的序列在Genebank中与三磷酸甘油醛脱氢酶(Glyceraldehyde-3-phosphate dehydrogease,GPD)基因的同源性达到85%,根据SL1序列设计引物,以黄伞菌丝体、原基、菇蕾、子实体菌柄、子实体菌盖的cDNA为模板进行PCR反应以验证其保守性,结果显示此片段在不同分化发育阶段表达量相同。The mixed primer was designed, and two fragments SL1 and SI2 were acquired by PCR templating of the eDNA of Pholiota adiposa （Fr.） quél mycelium. After reamplifying, purifying, cloning, measuring sequence and blasting in GeneBank, the result showed that identified SL1 fragment is similar to Glyeeraldehyde - 3 - phosphate dehydrogease （GPD） gene, and the homology is as high as 85% . The primer is designed on the basis of SL1, eDNA of different period including mycelium, anlage, bud and fruit body （stem and lid of mushroom） was extracted and treated by PCR to verifying the conservativeness. The result shows that the expression quantity of this fragment is uniform during different developing period of Ph adipose.

关 键 词：黄伞 三磷酸甘油醛脱氢酶基因 克隆 

分 类 号：S646[农业科学—蔬菜学]