急性排斥反应时移植肾组织中程序性死亡配体-1及其受体的表达  

Expression and significance of programmed death-ligand 1 and programmed death-1 in acute allograft rejection

作　　者：吴雄飞[1] 丁涵露[1] 张建国[2] 何娅妮[2] 李开龙[2] 

机构地区：[1]第三军医大学附属西南医院肾科,重庆400038 [2]第三军医大学附属大坪医院野战外科研究所肾内科

出　　处：《中华器官移植杂志》2005年第9期536-538,共3页Chinese Journal of Organ Transplantation

摘　　要：目的探讨急性排斥反应时移植肾组织中程序性死亡配体-1(PD-L1)及程序性死亡-1(PD-1)的表达及其与肾小管间质病理损害程度的关系。方法当肾移植患者发生急性排斥反应并经病理检查确认时,采取移植肾组织,以免疫组化和原位杂交染色法,观察肾组织中PD-L1和PD-1的表达,分析PD-L1阳性强度与肾小管-间质PD-1阳性细胞数、肾小管间质病理损害程度的关系。以正常肾组织为对照。结果急性排斥反应时,移植肾组织中的PD-L1及PD-1的表达较正常组织增多、增强(P<0.01);肾小管PD-L1阳性强度与肾间质PD-1阳性细胞数呈正相关,与肾小管间质病理损害程度呈负相关。结论急性排斥反应时,PD-L1及PD-1的表达增强,它们可能在肾小管间质病理损害中起重要作用。Objective To investigate the expression of programmed death-ligand 1 （PD-L1） and programmed death-1 （PD-1） in the kidney tissue of acute allograft rejection （AR） and the correlation between PD-L1 expression and PD-1 expression and the degree of tubulointerstitial lesions. Methods Immunohistochemistry and in situ hybridization were used to detect the expression of PD-L1 and PD-1 in renal tissue of normal control and AR confirmed by biopsy, Results Both PD-L1 and PD-1 expression were significantly higher in AR than in the normal renal tissue （P〈0.01）. The intensity of PD- L1 in renal tissue of AR showed a positive correlation with PD-1 expression and a negative correlation with the development of tubulointerstitial lesions. Conclusion Increased expression of PD-L1 and PD- 1 in renal tissue of AR have an important effect on the development of tubulointerstitial lesions.

关 键 词：移植物排斥 PD-L1 PD-1 肾移植 急性排斥反应 移植肾组织 程序性死亡 死亡配体 反应时 病理损害程度 

分 类 号：R699[医药卫生—泌尿科学]