蛋白质DNA混合微点阵和微流控芯片的整合  

Microarrays of DNA and Protein Integrated on Microfluidic Chip

作　　者：张志祥[1] 沈铮[2] 赵辉[3] 李宾[1] 宋世平[1] 胡钧[1] 林炳承[2] 李民乾[1] 

机构地区：[1]中国科学院上海应用物理研究所,上海201800 [2]中国科学院大连化学物理研究所,大连116023 [3]中国科学院上海微系统与信息技术研究所,上海200050

出　　处：《化学学报》2005年第18期1743-1746,共4页Acta Chimica Sinica

基　　金：国家自然科学基金(Nos.10335070;30470441);上海市纳米专项基金(No.55040213)资助项目

摘　　要：在活化的石英片上制作蛋白质和DNA微点阵,并可逆地将其与含有通道的多聚二甲基硅氧烷弹性橡胶封接在一起,使蛋白质和DNA微点阵组装在微通道列阵内;实现在微通道列阵内同时检测和分析蛋白质与DNA的功能.为了降低多聚二甲基硅氧烷弹性橡胶的疏水性,增强其生物相容性,实验通过多聚赖氨酸对多聚二甲基硅氧烷弹性橡胶的修饰,提高了它的亲水性,使溶液能够在微通道内顺畅地流通.实验表明,这种混合芯片能够提高检测速度和增加检测的信息量.To combine the advantages of high-throughput microarray and microfluidic chip technology, microarray was fabricated in channels. DNA and protein were arrayed on quartz slide, which was reversibly sealed by poly（dimethylsiloxane） （PDMS） slab containing pattern of channels. The hybridization carried out in channels indicated that target DNA and proteins were bound to their complementary DNA and protein probes specifically. In this method, PDMS conjugated with polylysine was contributed to imparting the wettability and decreasing unspecific adsorption of biomolecules. Our data demonstrated that the integrated chip speeded up the detection compared to microarrays, extended the parallel channels with serial properties and enhanced the throughput of probes and targets.

关 键 词：微点阵 微流控芯片 修饰 多聚赖氨酸 DNA 蛋白质 混合 聚二甲基硅氧烷 整合 同时检测 

分 类 号：Q51[生物学—生物化学]