几种生物碱基的反相高效液相色谱分离及人尿中尿嘧啶和腺嘌呤的测定  

Separation of Some Biological Bases by Reversed-Phase High Performance Liquid Chromatography and the Determination of Uracil and Adenine in Human Urine

作　　者：石义林[1] 韩金土[2] 刘锦春[3] 程介克[3] 

机构地区：[1]湖北咸宁教院化学系 [2]河南信阳师院化学系 [3]武汉大学化学系,武汉430072

出　　处：《分析科学学报》1996年第1期19-22,共4页Journal of Analytical Science

基　　金：湖北省自然科学基金资助课题

摘　　要：本文报道在Spherisorb C_(18)柱上,以H_3PO_4-KH_2PO_4溶液作流动相(pH4.0),同时分离和测定胞嘧啶、尿嘧啶、胸腺嘧啶、腺嘌呤和黄嘌呤5种生物碱基,用于人尿分析,标准加入回收率在92.5～105.5%之间.In this poper, a method for the separation and determination of cytosine, uracil, thymine,adenine and xanthine on a spherisorb C_(18) column with H_3PO_4-KH_2PO_4 solution (pH4.0) as a mobile phase is developed. The method was used for determination of uracil and adenine in human urine,and recoveries of 92.5～105.5% were obtained,

关 键 词：HPLC 生物碱基 尿 尿嘧啶 腺嘌呤 

分 类 号：Q526[生物学—生物化学] O652.63[理学—分析化学]