肝力克对肝纤维化大鼠肝组织ET-1mRNA、iNOSmRNA表达的影响  

EFFCT OF GANLIKE ON ET-1 mRNA and iNOS mRNA EXPRESSION IN LIVER TISSUE OF RATS WITH HEPATIC FIBROSIS

作　　者：陈芝芸[1] 严茂祥[1] 夏亮[1] 项柏康[1] 

机构地区：[1]浙江省中医院,杭州310006

出　　处：《中国中医药科技》2005年第5期277-278,280,共3页Chinese Journal of Traditional Medical Science and Technology

基　　金：浙江省教育厅资助项目No.19990360;浙江省中医管理局资助项目No.2000Y03

摘　　要：目的观察肝纤维化大鼠肝组织中ET-1mRNA、iNOSmRNA的表达及中药肝力克对其的影响,探讨肝力克治疗肝纤维化的可能作用机制。方法以40%CCl43ml/Kg体重皮下注射,连续9周,建立大鼠肝纤维化模型;将肝纤维化大鼠随机分为肝纤维化模型组、肝力克大、中和小剂量组,另设正常对照组。采用原位杂交法检测大鼠肝组织中ET-1和iNOS的mRNA表达情况。结果肝纤维化模型组大鼠肝组织ET-1mRNA、iNOSmRNA阳性表达均较正常对照组明显升高(P<0.01);应用肝力克能明显改善大鼠肝组织炎症活动度和纤维化程度,能明显下调肝纤维化大鼠肝组织iNOSmRNA表达,但对ET-1mRNA的表达无明显影响。结论ET-1、iNOS参与肝纤维化的发生发展,肝力克下调肝组织iNOSmRNA的表达可能是其抗肝纤维化作用的机理之一。Objective:To observe the effect of ganlike on ET-1mRNA and iNOS mRNA expression in liver tissue of rats with hepatic fibrosis and discuss the mechanism.Mthods:the model of rats′ hepatic fibrosis were induced by subcutaneous injection of CCl_4, then the rats in the treatment groups were given the different dosages of ganlike by stomach-perfusion,the rats in the groups of the model and the normal were given saline,the mRNA expression of ET-1 and iNOS in all liver tissues was detected by in situ hybridization method.Results:the mRNA expression of ET-1 and iNOS in the hepatic fibrosis were significantly higher than that of the normal group(P<0.01),after treated with ganlike,the severity of inflammation and fibrosis were obviously improved,and the expression of iNOS mRNA in hepatic fibrosis was downregulated.the expression of ET-1 mRNA showed no significance.Conclusion:ET-1 and iNOS participate in the occurrence and development of hepatic fibrosis,ganlike may downregulate the mRNA expression of iNOS in the liver tissues of hepatic fibrosis,which may be a possible mechanism of anti-hepatic fibrosis.

关 键 词：肝力克 肝纤维化 大鼠 肝组织 ET-1MRNA INOSMRNA 基因表达 中药制剂 

分 类 号：R285[医药卫生—中药学]