丝克高粱rDNA ITS区的RFLP分析  

rDNA ITS gene of silk sorghum by RFLP

作　　者：郭琼霞[1] 黄可辉[1] 虞赟[1] 吴珍泉[2] 

机构地区：[1]福建出入境检验检疫局,福建福州350001 [2]福建农林大学生物防治研究所,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2005年第3期349-352,共4页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家质量检验检疫监督总局2002年科技项目(IK2002059)

摘　　要：对检疫性杂草丝克高粱及其5个同属近似种假高粱、明福1号、拟高粱、苏丹草和黑高粱的rDNA内转录间隔区(ITS)进行PCR扩增及测序,并利用限制性内切酶XceⅠ对这些近似种的PCR产物进行酶切分析.结果表明:各个种间均有XceⅠ酶切位点,其中假高粱、明福1号、拟高粱、苏丹草及黑高粱5个种的酶切图谱完全一致,而丝克高粱的酶切图谱与其5个同属近似种的酶切图谱不同.假高粱、明福1号、拟高粱、苏丹草及黑高粱的rDNA PCR扩增产物可以切出4条带,大小约为670、530、190、150 bp;而丝克高粱只能切出2条带,大小约为670、190 bp,这与其5个同属近似种在rDNA ITS区有2个XceⅠ酶切位点,而丝克高粱只有1个酶切位点的结果相一致.因此,利用XceⅠ就可以把丝克高粱与其5个近似种区分开.Internal transcribed spacer （ITS） sequences of rDNA from silk sorghum as a quarantine weed and related species were obtained. By restriction endonuclease digestion, restriction enzyme Xce Ⅰ cut the PCR product. The sequence alignment showed that only 16 base pairs differences were found in ITS （ITS1,5.8S RNA, ITS2）. It indicated that rDNA ITS region was relatively stable among the intrapopulations of 6 related species. The fragments of rDNA ITS-PCR were digested with Xce Ⅰ. The difference between silk sorghum and its related species was found according to the pattern of digestion by Xce Ⅰ . That is, only two bands in 670, 190 bp were observed in the lanes of silk sorghum while the four fragments in 670,530, 190 and 150 bp for other related species. The results were consistent with the fact that S. halepense, S. propinguum, S. propinguum （ Mingfu No. 1 ）, S. almum and S. sudanense each had two selective identify sites of PmaC Ⅰ while S. silk had one site, Therefore, silk sorghum can be distinguished by the PCR- RFLP method established in this study.

关 键 词：丝克高粱 内转录间隔区 限制性片段长度多态性分析 分子鉴别 

分 类 号：S41-31[农业科学—植物保护]