针刺对部分背根切断备用背根节生长的影响  

Effect of Partial Ganglionectomy and Acupuncture on Culturing Spared DRG in vitro

作　　者：王特为[1] 王廷华[1] 周雪[2] 张连双[2] 徐新运[2] 

机构地区：[1]昆明医学院神经科学研究所,昆明650031 [2]四川大学华西基础医学与法医学院组织胚胎学与神经生物学教研室

出　　处：《四川大学学报（医学版）》2005年第5期630-633,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30260125);云南省教委基金(批准号0012051);纽约中华医学基金(CMB00-722)资助

摘　　要：目的探讨针刺对部分背根切除备用背根节(DRG)生长的影响.方法健康成年雄猫10只.随机分为正常对照组、备用根模型组及针刺备用根模型7 d组(摘除双侧L1～L5, L7～S2 DRG, 保留L6 DRG为备用背根,其中针刺一侧备用根支配区的两组穴位即为针刺组,非针刺侧为备用根模型组),每组5只.两组动物在无菌条件下取出双侧L6 DRG进行体外培养.分别于1、3、5、7 d 250倍光镜下随机测量20个视野的细胞突起长度.用抗神经元特异性烯醇化酶(NSE)抗体行免疫组织化学ABC法染色对神经元进行鉴定.结果培养的DRG细胞经抗NSE抗体行免疫细胞化学ABC法染色,可见95%以上呈NSE阳性反应,且为典型的体外培养的DRG神经元.在相应时间段正常组DRG神经元的神经突起长度小于备用根组(P<0.05)、备用根组小于针刺组(P<0.05).结论部分背根切断可致备用DRG神经元突起再生活性增强,针刺备用根所支配的穴位后可进一步促进备用DRG神经元的突起再生.Objective To explore the effect of partial dorsal root rhizotomy and Acup on culturing dorsal root ganglion（DRG） in vitro. Methods Ten adult cats were divided into 2 groups： normal control group; Acup spared DRG 7 d group, in which bilateral L1-L5, L7-S2 DRG were removed, and L6DRG were spared; then unilaterally two sets of acupoints [Zusanlily（St. 36） and Xuanzhong（G. B. 39） ; Futu（St. 32） and Sanyinjiao（Sp. 6） located in the distribution area of spinal nerve L6] were electro-stimulated alternatively 30 min everyday by electroneedling. Five cats were used in every group. Bilateral L6 DRGs of every group were taken out on the condition of asepsis and were cultured respectively in vitro. Cultures were terminated after day 7. Then the cultured cells were stained under the same condition using specific NSE （1 ： 200） antibody, a neuron-specific marker, by the immunohistochemistry ABC method. The neurite length was measured by micro-measured ruler in upside-down light microscope on the 1st, 3rd, 5th, 7th day. Results Immunocytochemical staining revealed that over 950% cells were NSE positive cells which were the typical neuron of DRG in vitro; on the 1st, 3rd, 5th, 7th day, the average neurite length of the normal group was shorter than that of the spared DRG group （P〈0.05）, and the spared DRG group＇s was shorter than the Acup group＇s at each time stage （P〈0. 05）. Conclusion These results indicated that DRG had plasticity and acupuncture probably promoted the plasticity, which were probably in close relation with the spinal plasticity.

关 键 词：针刺 DRG培养 DRG可塑性 脊髓可塑性 

分 类 号：R245[医药卫生—针灸推拿学]