TGF-β_3在中子及γ线照射小鼠肠道损伤中的作用研究  

Role of TGF-β_3 in injury of mouse intestines induced by neutron and γ rays irradiation

作　　者：彭瑞云[1] 高亚兵[1] 陈浩宇[1] 吴小红[1] 付凯飞[1] 王水明[1] 王德文[1] 胡文华[1] 马俊杰[1] 王晓民[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850

出　　处：《军事医学科学院院刊》2005年第4期341-343,共3页Bulletin of the Academy of Military Medical Sciences

基　　金：全军"十五"指令性课题(01L019);国家自然科学基金资助项目(30370438)

摘　　要：目的:比较研究小鼠经中子及γ线照射后肠组织中TGF-β3的表达及意义。方法:350只二级雄性BALB/c小鼠,经不同剂量的中子和γ线照射,于照后6、12h,1、2、3、4、5、7、10、14、21及28d分批活杀,采用免疫组织化学和图像分析等技术,定量研究TGF-β3在肠组织中的动态变化规律。结果:2.5Gy照后2d内,TGF-β3表达进行性减少,阳性部位见于绒毛上皮细胞和隐窝细胞浆内;3~7d明显增加,5d达高峰;7d后逐渐恢复至正常水平。4.0,5.5Gy中子及12Gyγ线照射后4d内,TGF-β3表达进行性减少;γ线5.5Gy照后6h,TGF-β3表达反应性增加;12h^1d减少,2~5d增多,3d达高峰;5d后逐渐恢复至正常水平。结论:中子和γ线照射后,肠内源性TGF-β3的表达具有不同的变化规律,参与了肠放射损伤及修复的病理过程。Objective：To study comparatively the changes and significance of transforming growth factor beta-3 （TGF-β3 ） in the injury of mouse intestines induced by neutron and γ rays irradiation. Methods： A total of 350 male BALB/c mice were used, irradiated with neutron and γ rays, sacrificed at 6 and 12 hours, 1,2, 3, 4, 5, 7, 10, 14, 21 and 28 days respectively after irradiation. The TGF-β3 in the mouse intestinal tissue was detected by means of immunohistochemistry and image analysis. Results ： The expression of TGF-β3 in the cytoplasm of mucosal epithelial cells and crypt cells decreased obviously within 2 days, increased 3-7 days, peaked 5 days and recovered 7 days after 2.5 Gy neutron irradiation. TGF-β3 decreased progressively within 4 days after 4.0, 5.5 Gy neutron and 12 Gy γ rays irradiation. It increased at 6 hours and decreased in 12 hours to 1 day, increased again 2-5 days, peaked 3 days and recovered 5 days after 5.5 Gy γ rays irradiation. Conclusion： The expression of endogenous TGF-β3 has different regularities of change after neutron and γ rays irradiation and plays an important role in the pathologic courses of intestinal damage and recovery.

关 键 词：中子 Γ线 小鼠 肠 TGF-Β3 免疫组织化学 图像分析 

分 类 号：R818[医药卫生—放射医学]