多重PCR检测性传播生殖器溃疡性疾病病原体方法的研究  

Study on the Detection of Agents Causing Genital Ulcer Disease by Multiple Polymerse Chain Reaction (PCR)

作　　者：刘爱英 尹跃平[2] 孙建方[2] 陈祥生[2] 余艳华 

机构地区：[1]煤炭总医院皮肤性病科,北京100028 [2]中国医学科学院,中国协和医科大学皮肤病研究所,江苏南京200042

出　　处：《中国皮肤性病学杂志》2005年第9期530-532,共3页The Chinese Journal of Dermatovenereology

摘　　要：目的建立多重PCR用于快速检测引起性传播生殖器溃疡性疾病(GUD)常见病原体。方法根据各病原体特异性基因序列,即单纯疱疹病毒I和II型(HSV1和HSV2)的DNA聚合酶基因、梅毒螺旋体47KD膜蛋白基因、沙眼衣原体的主要外膜蛋白基因(omp1/ompb)及杜克雷嗜血杆菌16SrRNA基因序列,自行设计4对特异寡核苷酸引物,建立同时检测4种病原体DNA的多重PCR。结果各特异性引物仅扩增相应的病原体DNA。其他几种生殖道常见菌及人基因组DNA不被上述引物扩增。检测了51例临床标本,多重PCR检查结果与暗视野显微镜检查梅毒螺旋体和培养法检查HSV结果进行比较,均有较好的一致性。结论多重PCR检测4种引起GUD常见病原体DNA具有较好的特异性,可为GUD患者提供敏感、快速和准确的诊断手段。Objective To establish multiple PCR assay for simultaneous detection of agents causing genital ulcer disease （GUI））. Methods According to specifie gene sequence of four agents：herpes simplex virus （HSV） DNA Polymerse gene,treponema pallidum（TP） tpp47 gene,Chlamydia traehomatis（CT） ompl/ompb gene,Haemophilus ducreyi （HD）16s rRNA gene,four sets of specific primers were designed and a multiple PCR （M-PCR） assay was developed to detect 4 agents in one test, Results The target DNA of 4 agents were specifically amplified by their specific primers, The DNA of other several common microbe of genital tract and human gene were not amplified by four sets of primers, 51 clinical samples were detected by dark-field microscopy, HSV culture and M-PCR,there was a better agreement between M-PCR and dark-field microscopy （ Kappa = 0. 73 ） , M-PCR and HSV culture （ Kappa = 0.43）, Conclusion Primary study indicated that the M-PCR assay can simultaneously,specifically,rapidly detect four agents of genital ulcer disease.

关 键 词：多重PCR 性传播生殖器溃疡 病原体 

分 类 号：R759[医药卫生—皮肤病学与性病学]