HPLC测定强肾胶囊中大黄素的含量  

Determination of the Rheum Emodin in Qiangshen Capsule by HPLC

作　　者：张玲[1] 赵建平[1] 

机构地区：[1]山西省中医药研究院,山西太原030012

出　　处：《山西中医学院学报》2005年第3期45-46,共2页Journal of Shanxi College of Traditional Chinese Medicine

摘　　要：目的:对强肾胶囊中大黄所含主要化学成分大黄素进行测定含量。方法:采用高效液相法,色谱柱为YW G C18柱(250m m×4.6m m,10μm);甲醇-0.1%磷酸溶液(85∶15)为流动相,流速为1.0m l/m in,检测波长为254nm。结果:方法学考察结果表明,该研究建立的分析方法有较好的重现性和稳定性;测定方法的平均回收率为99.65%(RSD=1.6%)。结论:H PLC法简便、快速、准确,可用于强肾胶囊的质量控制。Objective： To establish a determination method for ephedrine hydrochloride and pseudo ephedrine hydrochloride in Qiangshen Capsule. Methods： HPLC with YWG C18（250 mm×4.6 mm 10 μm） column was used. Methanol- 0. 1% H3PO4（85：15）was used. The flow rate was 1.0 ml/min and the detection wavelength was at 254 nm. Results： The methodological evaluation show that this method had a good repeatability and reproducibility. The average recovery was 99. 65 % （RSD = 1.6 % ）. Conclusion： This method is simple, quick, accurate and with good reproducibility and can be used for the quality control of Qiangshen Capsule.

关 键 词：HPLC法 测定方法 强肾胶囊 大黄素 含量检测 中药制剂 质量控制 

分 类 号：R286[医药卫生—中药学] R284[医药卫生—中医学]