β-琼脂糖酶(AgaA7)基因的克隆与表达  

CLONING AND EXPRESSION OF β-AGARASE(AgaA7)

作　　者：王广慧[1] 张明[2] 魏群[1] 

机构地区：[1]北京师范大学生命科学学院,北京100875 [2]吉林农业科学院,吉林省公主岭136100

出　　处：《北京师范大学学报（自然科学版）》2005年第4期415-419,共5页Journal of Beijing Normal University(Natural Science)

摘　　要：为研究重组β琼脂糖酶(AgaA7)基因的表达产物及其生物活性,根据GeneBank中β琼脂糖酶(AgaA7)基因的编码序列合成此基因后,将其克隆到表达载体PQE30中,测序结果证明合成基因序列正确,未改变读码框.然后将此表达载体转入E.coliM15中进行诱导表达.纯化后的表达产物经SDS-PAGE检测其相对分子质量并鉴定其生物活性.结果表明:其相对分子质量约为5.0×104,与预期相符,具有生物活性.In order to study the exprssion product of recombinant β-agarase （AgaA7） gene and its bioactivities, the full β-agarase（AgaA7） sequence is synthesized according to the GeneBank. Then it is inserted into expression vector PQE30. Its sequence is verified and the reading frame is proved to be correct. The recombinant plasmid is transformed into E. coli M15. The molecular mass of the purified expression product is examined by SDS-PAGE and its bioactivities are proved.

关 键 词：β-琼脂糖酶(AgaA7) 基因重组 蛋白表达 生物活性 

分 类 号：Q785[生物学—分子生物学]