高血压大鼠ACE2的表达及其与一氧化氮相关性分析  

Angiotensin-Converting Enzyme 2 Expression and Nitric Oxide in Spontaneously Hypertensive Rats

作　　者：钟久昌[1] 黄东阳[2] 余细勇[1] 

机构地区：[1]广东省心血管研究所广东省人民医院医学研究中心,广东广州510080 [2]汕头大学医学院分子生物学中心,广东汕头515041

出　　处：《高血压杂志》2005年第9期564-567,共4页Chinese Journal of Hypertension

基　　金：国家自然科学基金资助(30370340)

摘　　要：目的探讨血管紧张素转换酶2(ACE2)在自发性高血压大鼠(SHR)中的表达情况并对其与血清一氧化氮(NO)水平作相关性分析。方法分别采用实时荧光定量PCR,Northern印迹以及免疫印迹技术测定心、肾组织中ACE2的mRNA与蛋白表达情况,同时用硝酸还原酶法检测血清NO含量。结果与WKY对照组相比,SHR大鼠心、肾组织中ACE2的mRNA和蛋白表达均明显下降(ACE2/GAPDH mRNA拷贝数比值为:心脏0·043±0·012vs1.291±0·619;肾脏0·051±0·016vs0·914±0·433,P均<0·01;蛋白相对OD值为:心脏0·729±0·046vs1±0·053;肾脏0·734±0·063vs1.005±0·05,P均<0·01),同时出现血清NO浓度下降[(24.3±8.0vs78.4±27.9)μmol/L,P<0·01)。相关分析发现,大鼠血清NO水平与心、肾组织中ACE2/GAPDH的mRNA拷贝数比值呈正相关(r=0·610,0·521,P均<0·05),而与血压水平则呈负相关(r=-0·656,P<0·05)。结论SHR大鼠心、肾组织中ACE2的mRNA和蛋白表达均明显下降,很可能与体内NO水平降低及血压上升有关。特异性影响ACE2转录和表达的药物将对高血压病的防治有重要的临床应用价值。Objective To study the expression of angiotensin-converting enzyme 2（ACE2） in spontaneously hypertensive rats （SHR） and its relavance to serum levels of nitric oxide（NO）. Methods mRNA and protein expression of ACE2 in heart and kidney have been examined by real-time polymerase chain reaction, Northern blot, and Western blot methods. In addition, serum NO levels were determined after reduction with nitrate reductase. Results ACE2 mRNA and protein expression were markedly decreased in heart and kidney in SHR compared with WKY rats （the copy ratio of ACE2/GAPDH mRNA： Heart 0. 043±0. 012 vs 1. 291±0. 619; Kidney 0. 051±0. 016 vs 0. 914±0. 433, P〈0.01, respectively; the relative OD valueof protein： Heart 0.729±0.046 vs 1.0±0.053; Kidney 0.734±0.063 vs 1. 005±0.05, P〈 0.01, respectively）. The decreased expression of ACE2 were accompanied by reduction of serum NO [（24.3 ± 8.0 vs 78.4 ± 27.9）μmol/L, P〈0.01]. Serum NO are positively correlated to the copy ratio of ACE2/GAPDH mRNA in heart and kidney （ r= 0.610, 0.521, P〈 0.05, respectively） and negatively correlated to blood pressure（r=-0. 656,P〈0.05）. Conclusion Significant downregulation of mRNA and protein expression of ACE2 was found in heart and kidney in SHR, which is likely related to the reduction of NO and the subsequent rise of blood pressure.

关 键 词：血管紧张素转换酶2 高血压 一氧化氮 自发性高血压大鼠(SHR) 血清一氧化氮(NO) ACE2 相关性分析 NORTHERN印迹 表达及 实时荧光定量PCR 

分 类 号：R544.1[医药卫生—心血管疾病]