柴油车尾气颗粒物提取物对V79细胞DNA损伤研究  

Study on DNA damage caused by diesel exhaust particles extracts in V79 cell

作　　者：刘文彪[1] 董胜璋[1] 林忠宁[1] 胡前胜[1] 蔡承铿[1] 

机构地区：[1]中山大学公共卫生学院预防医学系,广东广州510080

出　　处：《毒理学杂志》2005年第3期199-201,共3页Journal of Toxicology

摘　　要：目的研究柴油车尾气颗粒物有机提取物(DEPE)对中国仓鼠肺成纤维细胞(V79)的DNA损伤及作用途径。方法通过单细胞凝胶电泳试验检测DEPE致细胞DNA单链、双链断裂及DNA蛋白质交联水平,并用抗氧化剂N乙酰半胱氨酸(NAC)作干预试验,探讨其损伤机制。结果DEPE能致细胞DNA单链及双链断裂,在500～1000μgmL能引起一定程度的DNA蛋白质交联,NAC可降低DEPE致DNA损伤。结论在一定剂量下,DEPE可致细胞DNA单链、双链断裂及DNA蛋白质交联,氧化损伤是导致DNA损伤的原因之一。Objective To study the DNA damage and its way in V79 cell caused by diesel exhaust particles extracts（DEPE）. Methods Detected the DNA damage with single-cell gel electrophoresis（SCGE） ,and the V79 cell were pretreated with N-acetylcysteine （NAC） to explore the way of DNA damage. Results It showed that single strands break （SSB） and double strands break （DSB） were observed in V79 cell exposed to DEPE. Moreover, DEPE also could induce DNA-protein crosslinks （DPC）at dosage of 500 and 1 000 μg/mL;and pretreatment of NAC could reduce the toxicity of DEPE to the cells. Conclusion DEPE could cause V79 cell three types DNA damage,including SSB, DSB and DPC,and the DNA damages might be related to oxidative stress.

关 键 词：柴油车尾气 有机提取物 DNA 氧化损伤 

分 类 号：R994.6[医药卫生—毒理学]