转基因SOD,POD高表达烟草幼苗抗热性研究  

Heat Resistance Increases in Seedlings of Transgenic Tobacco Lines with SOD,POD Genes

作　　者：刘明求[1] 刘齐元[1] 丁小维[1] 侯思名[1] 刘飞虎[1] 

机构地区：[1]云南大学生命科学学院,云南昆明650091

出　　处：《云南农业大学学报》2005年第5期620-623,共4页Journal of Yunnan Agricultural University

基　　金：云南省人才基金资助项目

摘　　要：生理高温(38±1)℃处理转基因烟草品系及非转基因品系的幼苗,测定其SOD,丙二醛、可溶性糖含量、电导率和生物量等指标。结果表明,转基因品系SOD的活性更强,丙二醛的含量更低,生物量受到的影响更小。由此认为,转基因烟草品系幼苗比非转基因品系的抗热性更强,其中转基因POD高表达烟草品系的抗热性最强。研究结果表明通过转基因方法提高植物的抗热性是可行的。Seedlings of transgenic tobacco and non-transgenic tobacco lines were treated with physiological high temperature（38±1 ）℃ for 3 d or 6 d. SOD activity, malondialdehyde content, soluble sugar content, electrical conductivity and biomass per plant were measured . While treated with physiological high temperature, SOD activities and biomass per plant were higher, malondialdehyde contents were lower in seedlings of transgenic tobacco lines than that in the non-transgenic line. The results indicated that heat resistance of transgenic tobacco lines was higher than that of the non-transgenic line. The line of transgenic tobacco with POD genes had the highest heat resistance. So it is possible to increase the heat resistance of plant by transgenic method.

关 键 词：生理高温 转基因烟草 抗热性 SOD POD 

分 类 号：S572.034[农业科学—烟草工业]