番茄晚疫病菌的分离、扩繁及人工接种研究  

Studies on Isolation,Propagation and Inoculation of Phytophthora infestans on Tomato

作　　者：吴涛[1] 朱海山[1] 

机构地区：[1]云南农业大学园林园艺学院,云南昆明650201

出　　处：《云南农业大学学报》2005年第5期655-658,661,共5页Journal of Yunnan Agricultural University

基　　金：云南省"十五"攻关项目(2001NG21)

摘　　要：研究了叶片诱导法、薯片诱导法分离番茄晚疫病菌及其在15%T-Rye,Oat,PDA这3种培养基上的扩繁效果,同时应用苗期接种、离体叶片接种两种方法,用生理小种T1,2,3的不同孢子囊悬浮液浓度对抗、感番茄品种进行人工接种研究。结果表明:(1)叶片诱导法容易分离到菌株;(2)15%T-Rye培养基最适合做扩繁培养基,病原菌在其培养基上生长最好,产孢量最多;(3)以2 000个/mL的孢子囊悬浮液接种番茄离体叶片后,根据第5 d的发病情况即可鉴别品种抗性。The results of isolation of Phytophthora infestans on tomato were studied by potato disc and in vitro leaf. The selected mediums of propagation were tomato juice-rye medium, oat medium and PDA medium respectively. The different concentrations of inoculation sporangia of P. infestans were 0, 2 000,4 000,6 000,8 000,10 000/mL on young plants and 0,1 000,2 000,3 000,4 000,5 000,6 000/ mL on detached leaves of tomato. The results showed that （ 1 ） it was easier to get P. infestans by in vitro leaf than by potato disc; （2） the tomato juice-rye medium was the best one among the three media ; （ 3 ） the most proper concentration was 2 000 sporangia per milliliter to distinguish different kind of resistant variety by detached leaf.

关 键 词：番茄 晚疫病菌 分离 扩繁 人工接种 

分 类 号：S436.412.12[农业科学—农业昆虫与害虫防治]