产ESBLs肠杆菌科细菌对氨基糖苷类抗生素耐药机制研究  

Aminoglycosides resistance mechanisms of extended spectrum β-lactamases-producing Enterobacteriaceae

作　　者：段建春[1] 吕晓菊[1] 赵燕[1] 冯萍[1] 俞汝佳[1] 宗志勇[1] 高燕渝[1] 熊亚莉[1] 

机构地区：[1]四川大学华西医院感染性疾病中心四川大学生物治疗国家重点实验室,成都610041

出　　处：《中国抗生素杂志》2005年第9期555-558,共4页Chinese Journal of Antibiotics

摘　　要：目的研究产超广谱β-内酰胺酶(ex tended spectrum beta-lactam ases,ESBL s)肠杆菌科细菌对氨基糖苷类抗生素的耐药分子机制。方法采用PCR方法对耐氨基糖苷类抗生素的产ESBL s菌株进行氨基糖苷类耐药机制研究,并同时对钝化酶基因DNA测序,进行网上基因相似性检索。结果20株耐氨基糖苷类的产ESBL s肠杆菌科细菌中存在氨基糖苷钝化酶基因,分别有aac(3)-I、aac(3)-II、aac(6′)-I、aac(6′)-II、ant(2")-I和aph(3)′-V I,以aac(3)-I、aac(3)-II和aac(6′)-I为主。部分菌株同时可含有多种钝化酶基因。DNA测序分析结果与国外报道已知相应氨基糖苷钝化酶DNA测序结果一致。结论产ESBL s肠杆菌科细菌中普遍存在氨基糖苷钝化酶,四川大学华西医院分离得到的氨基糖苷类耐药的产ESBL s肠杆菌科细菌的钝化酶基因型主要是aac(6′)-I、aac(3)-II、aac(3)-I和ant(2")-I。Objective To investigate ESBLs-producing Enterobacteriaceae strains. the molecular mechanisms of the aminoglycoside-resistance in Methods The nucleotide sequence of aminoglycoside-modifying enzyme genes in ESBLs-producing plus aminoglycoside-resistant strains amplified by PCR were detected by automatic sequencer, and compared with relative DNA sequencing reported by others in Internet GenBank through Blastx searching. Results Among 20 aminoglycoside-resistant plus ESBLs-producing strains, aminoglycoside-modifying enzyme genes have been found. They were aminoglycoside acetyltransferase （3）-Ⅰ gene [aac（3）-Ⅱ, aminoglycoside acetyltransferase （3）-Ⅱ gene [aac（3）-Ⅱ], aminoglycoside acetyltransferase （6＇）-Ⅰ gene [aac （6＇）-Ⅰ] ＇ aminoglycoside acetyltransferase （6＇）-Ⅱ gene [aac （6＇）-Ⅱ], aminoglycoside adenyltransferase （2＂）-Ⅰ gene [ant （2＂）-Ⅰ] and aminoglycoside phosphoryltransferase （3＇）-Ⅵ gene [aph （3＇）-Ⅵ]. The DNA sequences of aminoglycoside-modifying modifying enzymes genes were in concordance with other aminoglycoside-modifying enzyme genes refered in Internet GenBank. Conclusions Aminoglycoside-modifying enzyme were commonly existed in ESBLs-producing strains of Enterobacteriaceae. The genes encoding aminoglycoside-modifying enzymes were mainly aac（6＇）-Ⅰ, aac（3）-Ⅱ, aac（3）-Ⅰand ant（2″）-Ⅰ.

关 键 词：肠杆菌科细菌 超广谱Β-内酰胺酶 氨基糖苷类钝化酶 PCR 序列测定 

分 类 号：R378.2[医药卫生—病原生物学]