展示肿瘤特异性抗原表位的噬菌体在小鼠体内引起的细胞免疫变化  

Filamentous bacteriophage displayed tumor-specific antigen epitope(MAGE-A1) induced cellular immune in mice

作　　者：房金波[1] 赵连贵 宋金娜[1] 高瑞娟[1] 王丽[1] 

机构地区：[1]东北师范大学生命科学学院,吉林长春130024 [2]白城市教育学院,吉林白城137000

出　　处：《东北师大学报（自然科学版）》2005年第3期69-72,共4页Journal of Northeast Normal University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(30040014);吉林省科学技术厅国际合作基金资助项目(20010710)

摘　　要：以含有肿瘤特异性抗原表位的杂合型噬菌体(TSPA-M-A1)作为抗原,免疫C57BL/6J小鼠,观察免疫4周后小鼠的细胞免疫变化.结果表明:抗原TSPA-M-A1在小鼠体内能够提高T淋巴细胞对ConA刺激的反应性,使NK细胞杀伤活性明显增高,产生了特异性T淋巴细胞(CTL)反应,引起了较强的迟发型超敏反应.这些结果为肿瘤的免疫治疗提供了实验依据.The C57BL/6J mice were immunized with the purified hybrid phage, which displayed the minor - specific antigen epitope MAGE - Al161 -169 （TSPA - M - A1 ）. Tested the proliferation of T lymphocytes, NK kill rate, CTL response in the spleen by MTT, incorporating 3H- TdR and chromium- release assays. The result showed that the proliferation of T lymphocytes increased, the NK activity was enhanced obviously compared with control, elicited MAGE - A1 specific CTL responses in the spleen and delayedtype hypersensitivity（DTH）. These observations may provide a new strategy for cancer immunotherapy.

关 键 词：噬菌体展示 肿瘤特异性抗原 细胞免疫 CTL 

分 类 号：R392.12[医药卫生—免疫学]