斑鳠细胞色素b基因的初步研究  

Primary analysis of mitochondrial cytochrome b gene sequences of Chinese bagrid fish mystus guttatus

作　　者：任岗[1] 陈艺燕[1] 钱开诚[1] 陈迪[1] 章群[1] 

机构地区：[1]暨南大学水生生物研究所,广州510632

出　　处：《生态科学》2005年第3期237-239,共3页Ecological Science

基　　金：国家自然科学基金(项目编号:40106014)广东省自然科学基金(项目编号:000760)

摘　　要：斑鳠是珠江四大名鱼之一,目前资源量急剧下降,种质资源的保护迫在眉睫。测定了斑鳠线粒体细胞色素b 基因的全序列,该序列全长1 137 bp,其中T、C、A、G四种碱基含量分别为31.6%、25.7%、29.6%、13.1%,A+T 的含量(61.2%)显著高于G+C的含量(38.8%)。将测定序列(序列自编号:MGGD)与GenBank中的2个斑鳠序列(序列自编号:MGZJ、MGFJ)进行了比较,3个序列之间的序列差异为0.005-0.017,转换后的氨基酸序列间差异位点都仅为2 个,反应出细胞色素b基因作为功能蛋白质编码基因序列相对比较保守。鳠属鱼类线粒体Cytb基因的转换大于颠换。研究为斑鳠渔业种质资源保护和品种选育提供了遗传方面的资料,斑鳠的群体结构有待进一步深入研究。Mystus guttatus is one of four famous fishes in the Pearl River, but the population depression is repaidly rapidly in,ecent years, and fishing resource needs protection. 1137 nucleotides of mitochondrial Cytochrome b gene sequence（number： MGGD） have been obtained from Chinese bagrid fish Mystus guttatus, the contents of T, C, A, G of the gene are 31.6 %, 25.7%,29.6 %, 13.1%respectively. Respectively compared to other two sequences from GenBank（number： MGZJ,MGFJ）, the gene sequence divergence of M. guttatus among the three sequences is 0.005 to 0.017. The difference of amino acid sequences converted by gene sequences are all only 2 sites, so it shows that Cytb gene which acts as a functional gene is conservative. And value of transition is larger than value of transversions in Mystus fishes. The research is aimed to offer some genetic data of M. guttatus for fishing resource protection and genetic selection, and the structure of Mystus guttatus population need be further researched.

关 键 词：斑鱯 细胞色素B基因 序列差异 种质保护 

分 类 号：Q173[生物学—水生生物学]