川芎嗪对血管紧张素Ⅱ诱导的人α_1(I)胶原基因启动子活性的影响  

Effects of Tetramethylpyrazine on the Transcriptional Activity of α_1 Type I Collagen Promoter Induced by Angiotensin Ⅱ

作　　者：张冬梅[1] 秦英[1] 杨君[2] 于海[2] 牛福玲[1] 朱陵群[1] 王硕仁[1] 姜良铎[1] 周春华[2] 

机构地区：[1]北京中医药大学东直门医院,北京100700 [2]中国人民解放军海军总医院,北京100037

出　　处：《中国中医药信息杂志》2005年第9期21-22,共2页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学资助课题(30200371);北京市科技新星A类计划资助项目(H020820860130)

摘　　要：目的探讨血管紧张素Ⅱ(AngⅡ)对人α1(Ⅰ)胶原基因启动子活性的调控作用和川芎嗪干预的影响环节。方法将2.5kb长度的人α1(I)胶原基因启动子片段与氯霉素乙酰基转移酶(CAT)“报告基因”组成的重组体pCOLH2.5,采用脂质体法转染至培养的大鼠心肌成纤维细胞,分别加入AngⅡ和川芎嗪,ELISA法测定CAT活性。结果AngⅡ可明显促进pCOLH2.5片段的启动活性,川芎嗪能抑制AngⅡ的促pCOLH2.5启动活性(P<0.05)。结论川芎嗪能在转录水平上阻断AngⅡ诱导的人α1(I)胶原基因启动子活性增加,从而抑制I型胶原的合成,阻滞和延缓心肌纤维化的发生发展。Objective To study the roles of tetramethylpyrazine on regulation of collagen gene promoter induced by angiotensin Ⅱ. Methods Cardiac fibroblasts of rat transfected by pCOLH 2.5, which were composed of 2.5 kb length of α1 type Ⅰ collagen gene promoter segments and chloramphenicol acetyltransferase （CAT）, were added angiotensin Ⅱ and tetramethylpyrazine. The activity of CAT was measured by ELISA technique. Results Ang Ⅱ enhanced the activity of pCOLH2.5, tetramethylpyrazine could inhibited the transcriptional activity of the promoter increased by AngⅡ （P〈0.01）. Conclusion Tetramethylpyrazine could decrease the promoter activity of the human α1 （ Ⅰ ） gene, and inhibit the composition of collagen Ⅰ, against the myocardial fibrosis.

关 键 词：启动子 胶原 血管紧张素Ⅱ 川芎嗪 

分 类 号：R285.5[医药卫生—中药学]