桃红四物汤Ⅱ号抗血管生成作用及其对KDR/FLK-1表达的影响  

Effect of Taohong Siwu DecoctionⅡon Inhibiting the Agiogenesis and KDR/FLK-1 Expression

作　　者：徐晓玉[1] 王淑美[1] 陈伟海[1] 陈刚[1] 

机构地区：[1]重庆医科大学中医药学院,重庆400050

出　　处：《中药新药与临床药理》2005年第5期329-332,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：重庆市科委应用基础课题(编号渝科发技字[2002]18号)

摘　　要：目的探讨桃红四物汤(THSWD)Ⅱ号抗血管生成作用及其机理。方法以鸡胚绒毛尿囊膜(CAM)法检测THSWDⅡ号对新生血管的效应,以MTT法检测其对血管内皮细胞ECV304增殖有无抑制作用,并以免疫组化法观察该方对小鼠B16黑色素瘤血管内皮细胞KDR/FLK-1的表达和微血管密度(MVD)计数的影响。结果THSWDⅡ号1 g/mL、2 g/mL组CAM血管指数降低(P<0.01);THSWDⅡ号1 mg/mL、2 mg/mL组ECV304细胞吸光度值降低(P<0.01);THSWDⅡ号5 g/kg、10 g/kg组及THSWDⅡ号10 g/kg+环磷酰胺25 mg/kg组肿瘤重量、KDR/FLK-1的表达、MVD计数均降低(P<0.01)。结论THSWDⅡ号具有抗CAM血管生成、抑制ECV304细胞增殖、抑制小鼠B16黑色素瘤生长的作用。其抗肿瘤作用机制可能与抑制内皮细胞KDR/FLK-1的表达,继而抗血管生成有关。Objective To study the anti-agiogenesis effect of Taohong Siwu Decoction Ⅱ （THSD Ⅱ ） and to explore its mechanism. Methods Chicken Chorioallantoic Membrane （CAM） assay was used to study the anti-agiogenesis action of THSD Ⅱ in vivo. MTT assay was used to investigate its effect on the proliferation of human umbilical vein vascular endothelial cells ECV304 in vitro. Immunohistochemistry assay was used to observe its effects on the expression of KDR/FLK-1 in endothelial cells and the amount of micro-vessel density （MVD） in mice with B16 melanoma. Results THSD Ⅱ at the dosages of 1 g/mL and 2 g/mL could obviously inhibit agiogenesis in the CAM （P 〈 0. 01 ） , 1 mg/mL and 2 mg/mL decreased the proliferation of ECV304 significantly （P 〈 0. 01 ） ; THSD Ⅱ at the dosages of 5 g/kg, 10 g/kg and THSD Ⅱ 10 g/kg combined with cyclophosphamide 25 mg/kg significantly reduced the tumor weight as well as the expression of KDR / FLK-1 and the amount of MVD in mice （P 〈 0. 01）. Conclusion THSD Ⅱ has effect on inhibiting the CAM agiogenesis, ECV304 proliferation and B16 melanoma growth in mice. Its anti-tumor mechanism may be associated with inhibiting the expression of KDR / FLK-1 and reducing the MVD.

关 键 词：桃红四物汤Ⅱ号 抗血管生成 KDR/FLK-1 

分 类 号：R285.5[医药卫生—中药学]