黄连解毒汤传统汤剂与配方颗粒汤剂主要活性成分含量比较  

Comparison of the Main Components between Traditional Decoction and Dispensing Granule Decoction of Huanglian Jiedu Decoction

作　　者：雷鹏[1] 刘韶[1] 李新中[1] 付文基[1] 

机构地区：[1]中南大学湘雅医院药剂科,长沙410008

出　　处：《中药新药与临床药理》2005年第5期353-356,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：湖南省卫生厅中医药科技基金(编号:221201)

摘　　要：目的比较黄连解毒汤传统饮片汤剂与配方颗粒汤剂中盐酸小檗碱、巴马亭、药根碱、黄芩苷和栀子苷的含量。方法采用高效液相色谱法,色谱柱:Diamonsil C18柱(250×4.6 mm);流动相:水-甲醇-0.05%磷酸(梯度洗脱);柱温:35℃;流速1.0 mL/min;检测波长为345 nm、280 nm和238 nm。结果建立了同时对黄连解毒汤中的5个成分进行定量的测定方法,该方法快速、重现性好、灵敏度高。结论配方颗粒汤剂与传统饮片汤剂的色谱图基本一致,配方颗粒汤剂5个主要成分含量均比传统饮片汤剂高。Objective To compare the contents of berberine, palmatine, jatrorrhizine, baicalin and geniposide between traditional decoction and dispensing granule decoction of Huanglian Jiedu Decoction. Methods A HPLC method was carried out on Diamonsil C18 column （250 × 4.6 mm, 5 μm） . The mixture of H2O（A）, CH3OH （B） and 0.05 % H3PO4 solution （C） was used as the mobile phase for gradient elution. The column temperature was set up at 35 ℃ and the flow rate was 1 mL/min. The detection wavelength was 345nm for berberine, palmtine and jatrorrhizine, 280 nm for baicalin, and 238 nm for geniposide. Results This method can he used to determine 5 components in Huanglian Jiedu Decoction at the same time and the method was rapid, sensitive and accurate. Conclusion The HPLC chromatograms of traditional sliced decoction of Huanglian Jiedu Decoction are similar to the dispensing granule decoction. The contents of 5 main components in dispensing granule decoction are higher than those in traditional sliced decoction.

关 键 词：高效液相色谱法 黄连解毒汤 传统饮片汤荆 配方颗粒汤剂 盐酸小檗碱 盐酸巴马亭 盐酸药根碱 黄芩苷 栀子苷 

分 类 号：R284.1[医药卫生—中药学]